Imaging Analysis of Extracellular Dopamine Based on DNA Prism Probes
10.19756/j.issn.0253-3820.251101
- VernacularTitle:基于DNA三棱柱的细胞膜外多巴胺成像分析
- Author:
Tong-Kai ZHAN
1
;
Jia-Dong WU
;
Xiao-Fang GUO
;
Meng-Meng LIU
Author Information
1. 华东师范大学化学与分子工程学院,上海 200241
- Keywords:
DNA nanostructures;
Dopamine;
Imaging;
Aptamer;
Cell membrane
- From:
Chinese Journal of Analytical Chemistry
2025;53(8):1250-1258
- CountryChina
- Language:Chinese
-
Abstract:
Dopamine(DA)is an essential neurotransmitter.Research on its dynamic concentration fluctuations,particularly within the extracellular microenvironment,is crucial for elucidating neural signaling mechanisms and pathogenesis of related disorders.Among DA detection methods,fluorescence probe-based imaging offers advantages such as high sensitivity,high specificity,and non-invasiveness.However,the application of these probes for in situ DA monitoring has been limited by the challenges including probe internalization and difficulties in labeling across cells.Here,leveraging the programmability and high stability of DNA nanostructures,a DNA prism-based probe for imaging DA release at the single-cell level was designed and constructed.This probe utilized surface-modified cholesterol for efficient membrane anchoring and a DA aptamer-based"turn-on"sensing module to detect DA directly on the cell membrane.Using this probe,rapid DA release triggered by high K+stimulation was observed,with the released DA concentration increasing over time and peaking at 8 min post-stimulation.More notably,exploiting the probe's ability to simultaneously anchor to two cells,thereby forming cell clusters,revealed that the distribution of DA within the intercellular space was significantly higher than that in the cell body regions.This probe not only provided a method for DA imaging on the cell membrane but also laid a theoretical foundation for developing broader neurotransmitter detection platforms,holding significant scientific merit and application potential.
