Advances in Dual-response Adenosine Triphosphate Fluorescent Probes for Bioimaging
10.19756/j.issn.0253-3820.251144
- VernacularTitle:双响应三磷酸腺苷荧光探针用于生物成像的研究进展
- Author:
Qing-Yu XU
1
;
Xiang LI
;
Wei CAO
;
Zhi-Hua PENG
;
Jing-Bin ZENG
Author Information
1. 中国石油大学(华东)化学化工学院,化学品安全全国重点实验室,青岛 266580
- Keywords:
Multifunctional probes;
Fluorescence sensing;
Adenosine triphosphate;
Bioactive substances;
Bioimaging;
Review
- From:
Chinese Journal of Analytical Chemistry
2025;53(8):1213-1225
- CountryChina
- Language:Chinese
-
Abstract:
Adenosine triphosphate(ATP),as the core energy metabolism molecule in living systems,has dynamic changes closely related to fundamental physiological processes.To meet the urgent demand for spatiotemporal ATP detection in vivo and in situ,the development of highly sensitive multifunctional synchronous sensing fluorescent probes has become a recent research focus.These dual-function probes achieve fluorescence detection of dual targets by designing recognition sites for ATP alongside biological factors or microenvironment parameters such as reactive oxygen/nitrogen/sulfur species,metal ions,and enzymes,enabling physiological/pathological state correlation analysis through bioimaging.This paper systematically reviews recent advances in fluorescent probes for the collaborative detection of ATP and key biomolecules.It specifically examines probe construction strategies based on specific molecular recognition mechanisms(e.g.,metal coordination competition,electrostatic interactions,and host-guest recognition),multi-modal optical signal transduction mechanisms(ratiometric fluorescence,fluorescence lifetime,and photodynamic therapy),and their applications in pathological models such as oxidative stress monitoring,metal homeostasis imbalance,and enzyme activity co-detection.Finally,from the perspective of molecular probe engineering,current challenges and future research directions are proposed to provide methodological support for precise analysis of ATP-related life process regulation networks.
