In Vitro Metabolism Identification of 4-Fluoroisobutyrfentanyl by Ultra-High-Pressure Liquid Chromatography Q-Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometry
10.19756/j.issn.0253-3820.231423
- VernacularTitle:基于超高效液相色谱-四极杆/静电场轨道阱-高分辨质谱的4-氟异丁酰芬太尼体外代谢研究
- Author:
Meng LIU
1
;
Sen ZHAO
;
Jian HUANG
;
Hong ZHOU
;
Yao LIU
Author Information
1. 浙江警察学院,浙江省毒品防控技术研究重点实验室,杭州 310053;中国人民公安大学侦查学院,北京 100038
- Keywords:
4-Fluoroisobutyrfentanyl;
In vitro metabolism;
Metabolite;
Metabolic pathway;
Human liver microsomes
- From:
Chinese Journal of Analytical Chemistry
2025;53(7):1204-1212,后插1-后插3
- CountryChina
- Language:Chinese
-
Abstract:
Research on the metabolism of fentany analogues is significantly beneficial in prolonging the examination period and enhancing the efficiency of detection and can offer insights and guidance for examination and identification of illegally abused substances.In this work,human liver microsomes were used to identify the metabolites and metabolic pathway of 4-fluoroisobutyrfentanyl by ultra-high-pressure liquid chromatography Q-exactive HF hybrid quadrupole-orbitrap mass spectrometry(UHPLC-Q-Exactive-HF-MS).A total of 15 kinds of phase I metabolites were detected through various metabolic pathways,including N-dealkylation,hydroxylation,N-oxidation,carbonylation and reduction,and the N-oxidized,dihydroxylated and reduced metabolite were reported for the first time.Upon comparing the peak areas of metabolites,the N-dealkylated metabolite(M1),monohydroxylated metabolite on the phenethyl side chain(M5-4)and N-oxidized metabolite(M6)were recommended as the metabolic biomarkers of 4-fluoroisobutyrfentanyl.This study offered valuable insights and supporting evidence for investigation and identification of 4-fluoroisobutyrfentanyl in biological samples in forensic cases.
