Preparation of γ-Polyglutamic Acid Complex Medical Coating and Analysis of Its Antibacterial Properties
10.19756/j.issn.0253-3820.241358
- VernacularTitle:γ-聚谷氨酸复合物医用涂层的制备及抗菌性能分析
- Author:
Ke LUAN
1
;
Dong-Hua XU
;
Ming-Zhe WANG
;
Xu ZHANG
;
Qiu-Yan YAN
;
De-An SHI
;
Rui WANG
;
Heng-Chong SHI
;
Hong XU
Author Information
1. 湖北大学材料科学与工程学院,高分子材料湖北省重点实验室,武汉 430062;中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春 130022
- Keywords:
γ-Polyglutamic acid;
Quaternary ammonium salt;
Complex;
Antimicrobial;
Biocompatibility
- From:
Chinese Journal of Analytical Chemistry
2025;53(7):1196-1203
- CountryChina
- Language:Chinese
-
Abstract:
Medical device related infections caused by bacteria are common complications in clinical practice,and preventing bacterial colonization on the surface of medical materials is one of the important challenges in the medical field.Therefore,there is an urgent need to construct medical coatings that combine antibacterial properties and biocompatibility.In this study,a γ-polyglutamic acid(γ-PGA)complex with long-chain alkyl quaternary ammonium salts formed by electrostatic and hydrophobic interactions was prepared,which was insoluble in water but soluble in organic solvents(e.g.,ethanol),and was capable of constructing antimicrobial coatings on the surfaces of medical materials in a simple and efficient manner.The bactericidal effect of the coating was verified using viable bacteria counting experiments,and the results showed that the bactericidal rate of the coated thermoplastic polyurethane(TPU)membrane against Staphylococcus aureus was greater than 99.9%compared with that of the uncoated TPU membrane.In addition,a cytotoxicity assay was performed using the L929 fibroblast and cell proliferation detection kit(CCK-8),which showed that the survival rate of L929 fibroblasts on coated TPU was greater than 90%.Meanwhile,the hemolysis rate of coated erythrocytes was tested using fresh rabbit red blood cells(RBCs),and the hemolysis rate on the coated TPU surface was 1.5%.The above results indicated that the coating had good biocompatibility.The preparation method of medical antibacterial coating reported in this study provided a new idea for preventing bacterial infections related to implantable/interventional medical devices.
