Identification of Endogenous and Exogenous Testosterone and Dehydroepiandrosterone in Beef by Gas Chromatography Combustion Isotope Mass Spectrometry
10.19756/j.issn.0253-3820.241405
- VernacularTitle:气相色谱-燃烧-稳定同位素质谱法鉴别牛肉中内源性和外源性睾酮和去氢表雄酮
- Author:
Bo ZHAO
1
;
Huan-Huan CHEN
;
Wei CAI
;
Hai LU
;
Jie JIANG
;
Teng XING
;
Yan GAO
;
Li LIN
;
Wei LI
Author Information
1. 中国计量科学研究院化学计量与分析科学研究所,北京 100029
- Keywords:
Testosterone;
Dehydroepiandrosterone;
Beef;
Endogenous;
Exogenous;
Identifying
- From:
Chinese Journal of Analytical Chemistry
2025;53(7):1167-1176
- CountryChina
- Language:Chinese
-
Abstract:
Accurate identification of endogenous and exogenous substances in food,particularly in competition supplies,is crucial for ensuring food safety and fair competition,as well as for protecting the legitimate rights and professional reputations of athletes.Testosterone(T)and dehydroepiandrosterone(DHEA)are important steroid hormones that can stimulate protein synthesis,increase the number and volume of muscle cells,and promote muscle growth and recovery.Both are often illegally used in the animal husbandry industry to promote animal growth and improve meat quality.However,current research in this area remains limited,and identification technologies require further investigation.This study focused on the techniques for identifying endogenous and exogenous hormones including T and DHEA in beef.A Soxhlet extraction method was established,reducing the pretreatment cycle to 110 min while achieving high extraction efficiency,with recovery rates of 102.5%for T and 91.9%for DHEA,respectively.Based on this,a gas chromatography-combustion-isotope ratio mass spectrometry(GC-C-IRMS)method was developed for analyzing carbon isotopes in T and DHEA,eliminating the need for derivatization.By adding reference materials to the extract,simultaneous measurement of reference materials and target analytes was achieved.The measurement of caffeine reference material,T and DHEA was completed within 40 min,with a measurement repeatability of 0.02‰.Theδ13C values of T and DHEA in standard substances,which may serve as exogenous additives,were determined using elemental analysis-isotope ratio mass spectrometry(EA-IRMS).The results indicated an average δ13C value of-29.44‰±0.81‰(k=1)for 10 T standards and-30.86‰±0.87‰(k=1)for 14 kinds of DHEA standards.This approach effectively distinguished between endogenous sources and exogenous addition of these two hormones in beef,thereby providing vital technical support for the assurance and supervision of food safety.
