Simultaneous Determination of Perfluorooctanoic Acid and Perfluorooctane Sulfonate Isomers in Seawater by Online Solid Phase Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry
10.19756/j.issn.0253-3820.251104
- VernacularTitle:在线固相萃取-液相色谱-串联质谱法测定海水中全氟辛酸和全氟辛烷磺酸同分异构体
- Author:
Jun-Hui CHEN
1
;
Nan SHEN
;
Tong-Zhu HAN
;
Xiu-Ping HE
;
Xian-Guo LI
Author Information
1. 自然资源部第一海洋研究所,青岛市现代分析与近海生态环境安全保障重点实验室,青岛 266061
- Keywords:
Online solid phase extraction;
Per-and polyfluoroalkyl substances;
Laizhou Bay;
Isomers;
Seawater
- From:
Chinese Journal of Analytical Chemistry
2025;53(7):1146-1157
- CountryChina
- Language:Chinese
-
Abstract:
A new method was developed for simultaneous and efficient determination of linear perfluorooctanoic acid(n-PFOA)and linear perfluorooctane sulfonate(n-PFOS),and their typical branched isomers in seawater by online solid phase extraction-liquid chromatography-tandem mass spectrometry(Online SPE-LC-MS/MS).Only centrifugation of the seawater sample was required to remove the particulate matter,and then the seawater sample was directly injected and analyzed by online SPE-LC-MS/MS.An Eclipse Plus-C18 guard column was selected as SPE column for online enrichment of linear and branched isomers,and a F5 PFP column(150 mm×2.1 mm,2.7 μm)was used as the analytical column.Under the optimized experimental conditions,the separation and detection of all PFOA and PFOS linear and branched isomers could be completed within 20 min.The spiked recoveries of various target compounds ranged from 82.9%to 107.7%with detection limits and limits of quantification of 0.10-1.05 ng/L and 0.30-2.11 ng/L,respectively.The method was characterized by good precision(RSD≤9.10%)and linearity(R2≥0.990).Subsequently,linear and branched isomers of PFOA and PFOS in surface and bottom seawater samples collected from the Laizhou Bay of China were determined.The results showed that the detection rate of all the four branched PFOA isomers were 100%,with the highest average concentration of 25.85 ng/L found for 6m-PFOA,which accounted for 11.79%of the∑PFOA.For the five branched isomers of PFOS,the highest detection rate of 90.84%was found for 5m-PFOS.The highest average concentration of 0.64 ng/L was observed for 3m-PFOS,accounting for 19.88%of ∑PFOS.The proposed method provided an effective detection tool for qualitative and quantitative detection of PFOA and PFOS isomers in the marine aquatic environment.
