Design,Synthesis and Application of Peroxynitrite Anion Fluorescent Probe with Large Stokes Shift
10.19756/j.issn.0253-3820.251147
- VernacularTitle:具有大Stokes位移的过氧亚硝基阴离子荧光探针的设计、合成及应用
- Author:
Xin-Tong YANG
1
;
Xiao-Chun WANG
;
Cui-Ping MA
;
Liang-Wei ZHANG
;
Ling-Xin CHEN
Author Information
1. 青岛科技大学生物工程学院,青岛 266042
- Keywords:
Fluorescent probe;
Peroxynitrite anion;
Large Stokes shift;
Cell imaging;
Oxidative stress
- From:
Chinese Journal of Analytical Chemistry
2025;53(7):1118-1126,中插1-中插8
- CountryChina
- Language:Chinese
-
Abstract:
Peroxynitrite anion(ONOO-),which is originated from the reaction of nitric oxide(NO)and superoxide anion(O2-),plays a pivotal role in immune defense and signal regulation.Excessive levels of ONOO-may induce the occurrence of various diseases such as Alzheimer's disease,inflammation,cardiovascular disease and cancer,etc.Existing detection methods for ONOO-have limitations such as complexity,time consumption,and low cell biocompatibility.In this study,a novel fluorescent probe QFPD was developed using quinoline and diphenylphosphinamide as fluorescent keleton and recognition group,respectively.The strong oxidizing property of ONOO-triggerd the cleavage of phosphoramide bond,leading to fluorescence quenching and a visible color change of the solution from yellow to purple,thereby enabling"ON-OFF"type recognition of ONOO-.QFPD exhibited excellent characteristics including good selectivity and high sensitivity in fluorescence detection(Limit of detection of 1.37 μmol/L),large Stokes shift(130 nm),rapid response(10 min),and strong anti-interference ability.Additionally,QFPD demonstrated good biocompatibility and could realize real-time dynamic monitoring of endogenous ONOO-.Furthermore,QFPD was successfully applied to environmental toxicology research by visualizing the oxidative stress effects induced by microplastics polymethyl methacrylate(PMMA)and Hg2+exposure,which might be a novel tool for the mechanism research on oxidative stress associated with microplastic pollution and heavy metal exposure.
