Colorimetric Detection of Acetaminophen Based on Cerium-L-Cysteine Nanozyme
10.19756/j.issn.0253-3820.251022
- VernacularTitle:基于铈-L-胱氨酸纳米酶比色检测对乙酰氨基酚
- Author:
Yan-Yan WANG
1
;
Lan-Lan ZHENG
;
Hong CAO
;
Yang-Yang ZOU
;
Jun-Min CHANG
Author Information
1. 新疆医科大学药学院,乌鲁木齐 830011;嘉兴大学生物与化学工程学院,嘉兴 314001
- Keywords:
Cerium-L-cysteine;
Oxidase-like enzyme;
Synthesis;
Colorimetry;
Acetaminophen
- From:
Chinese Journal of Analytical Chemistry
2025;53(7):1097-1107
- CountryChina
- Language:Chinese
-
Abstract:
Nanomaterials(Ce-CS)with oxidase-like properties were synthesized in one step using L-cystine(CS)and ammonium cerium nitrate(CAN)as raw materials for detection of acetaminophen(APAP).The morphology,structure and elements composition of Ce-CS were characterized by scanning electron microscopy(SEM),energy dispersive X-ray spectroscopy(EDS),nitrogen adsorption specific surface area analysis(BET),X-ray diffraction(XRD),infrared spectroscopy(IR)and X-ray photoelectron spectroscopy(XPS).The Ce-CS with peroxidase-like activity could catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine(TMB)into blue oxided TMB(oxTMB),which had a significant absorption peak at 652 nm.Under the optimal catalytic conditions,i.e.,reaction temperature of 25℃(room temperature),pH=4.0,Ce-CS concentration of 1 mg/mL,and reaction time of 10 min,the catalytic mechanism and kinetics of Ce-CS were studied.When APAP existed in the reaction system,it could inhibit the peroxidase-like activity of Ce-CS,reduced the absorbance at 652 nm(A652),and the absorbance difference at 652 nm(ΔA652)had a good linear relationship with concentration of APAP in the range of 50-2000 μmol/L(R2=0.996),with a detection limit(S/N=3)of 0.1 μmol/L.This method was applied to detection of APAP in oral liquid and river water samples,with recoveries of 98.0%-102.0%,demonstrating the potential of Ce-CS as an oxidase substitute in drug analysis and environmental monitoring.
