Preparation and Characterization of Supramolecular Solvent and Its Extraction of Sulfonamide Antibiotics from Milk
10.19756/j.issn.0253-3820.241484
- VernacularTitle:超分子溶剂的制备与表征及其萃取牛奶中磺胺类抗生素的性能分析
- Author:
Jun-Qiang HAO
1
;
Zhi-Xin ZHANG
;
Yu-Heng LI
;
Fan-Di ZHAO
;
Yang-Yang ZHOU
;
Lei ZHAO
;
Wei-Jian ZHANG
;
Jia-Han XIE
;
Zhi-Bing WANG
Author Information
1. 长春工业大学化学与生命科学学院,长春 130012
- Keywords:
Sulfonamide antibiotics;
Dispersive liquid-liquid microextraction;
Supramolecular solvents;
High performance liquid chromatography;
Milk
- From:
Chinese Journal of Analytical Chemistry
2025;53(6):976-986
- CountryChina
- Language:Chinese
-
Abstract:
As a cheap and effective antibiotic,sulfonamides are often used in animal husbandry.However,their residues in animal-derived foodstuffs will threaten human health.Consequently,a high-performance liquid chromatography(HPLC)method integrated with supramolecular solvent microextraction was successfully established for simultaneous quantification of sulfonamide residues sulfachlorpyridazine,sulfamethoxazole,sulfamethoxypyridazine and sulfadimethoxine in milk matrices.This approach exhibited prominent characteristics of operational simplicity,environmental sustainability,and high extraction efficiency.The supramolecular solvents prepared by tributyl octylphosphine tetrafluoroborate and tetrahydrofuran were employed as extraction solvents.The analytes underwent isolation and concentration via dispersive liquid-liquid microextraction(DLLME)prior to quantitative determination using high-performance liquid chromatography(HPLC).The composition and microscopic morphology of the supramolecular solvent were characterized through a series of analytical techniques,including phase diagram,Fourier transform infrared spectroscopy,scanning electron microscopy,and inverted fluorescence microscopy and so on.The density and pH value of supramolecular solvents were determined.The extraction conditions were optimized through the one-factor experiments.The experimental results demonstrated that under the optimal extraction conditions,the four kinds of sulfonamide antibiotics exhibited excellent linearity within respective detection range(R2 ≥ 0.9998)and the limits of detection(LOD)were 0.67-1.45 μg/L.Compared with literature methods,this approach offered some advantages such as simplicity of operation and less reagent consumption,and could be used for analysis and detection of sulfonamide antibiotic residues in milk samples.The present method provided technical support for food safety regulation and paved a new way for the application of supramolecular solvents in the field of extraction and separation.
