Research on Discrimination of Degradation Levels in Shipwreck Archaeological Wood Based on Microscale Attenuated Total Reflection Fourier Transform Infrared Spectroscopy
10.19756/j.issn.0253-3820.241161
- VernacularTitle:基于显微衰减全反射傅里叶变换红外光谱的沉船考古木材降解程度判别研究
- Author:
Ren LI
1
;
Man-Li SUN
;
Li-Chao JIAO
;
Ya-Fang YIN
;
Zhi-Guo ZHANG
;
Fu-De TIE
Author Information
1. 西北大学文化遗产学院,文化遗产研究与保护技术教育部重点实验室,中国-中亚人类与环境"一带一路"联合实验室,西安 710127
- Keywords:
Shipwreck archaeological wood;
Attenuated total reflection Fourier transform infrared spectroscopy;
Wood cell wall;
Sparse partial least squares discriminant analysis;
Degradation level
- From:
Chinese Journal of Analytical Chemistry
2025;53(6):967-975
- CountryChina
- Language:Chinese
-
Abstract:
After the wooden shipwreck was recovered from the marine underwater environment,the wooden components undergo varying degrees of degradation,therefore,accurately determining the extent of degradation is a fundamental scientific issue for implementing effective preservation strategies.In this work,the wooden remains of Pinus massoniana excavated from the"Nanhai No.1"shipwreck(Southern Song Dynasty)were investigated and compared with the modern wood to discriminate the degradation levels of archaeological wood using attenuated total reflection Fourier transform infrared(ATR-FTIR)spectroscopy.The residual sugar content within wood cell walls was determined using a non-invasive automated microscale ATR-FTIR method to extract chemical information from the wood tangential section.Microstructural characterization of wood samples was conducted by super depth of field microscopy and scanning electron microscopy.FTIR spectral analysis was performed to evaluate the degradation state and elucidate changes in cellulose crystallinity.Finally,the combination of FTIR spectroscopy with the sparse partial least squares discriminant analysis(sPLS-DA)model facilitated the rapid discrimination of degradation levels in shipwreck archaeological wood,and the performance of the model was evaluated using receiver operating characteristic(ROC)curves and area under the curve(AUC).The results showed that the higher the degree of wood degradation,the lower the residual sugar content in the wood cell wall,and the residual glucose content of highly degraded wood was only 4.7％.Significant differences were observed in both the tangential section microstructure and FTIR characteristic absorption patterns across degradation levels,and as the degradation advanced,progressive cell wall loosening occurred alongside selective removal of polysaccharide components,and the relative lignin content was increased,resulting in an elevated A1509/A1370 ratio in FTIR spectra.The sPLS-DA model achieved excellent discrimination performance with AUC values exceeding 0.9,confirming that the combination of FTIR spectroscopy with sPLS-DA enabled accurate assessment of degradation levels in shipwreck archaeological wood.This study developed a rapid and accurate methodology for assessing degradation levels in shipwreck archaeological wood based on microscale ATR-FTIR spectroscopy,which would help to promote the accurate assessment of the preservation state of waterlogged wooden artifacts.
