Colorimetric Sensor for Sensitive Detection of Glutathione Based on Metal-Organic Framework Nanosheets
10.19756/j.issn.0253-3820.241402
- VernacularTitle:基于金属有机框架纳米片的比色传感器用于谷胱甘肽的高灵敏检测
- Author:
Yan WU
1
;
Jing-Lei RAN
;
Fang LIU
;
Huan ZHONG
;
Jin-Yang CHEN
Author Information
1. 南华大学化学化工学院,衡阳 421001;长江师范学院化学化工学院,重庆 408100;青岛科技大学,光电传感与生命分析教育部重点实验室,青岛 266042;广西医科大学,广西药物精准检测与筛选重点实验室,南宁 530021
- Keywords:
Colorimetric sensor;
Glutathione detection;
Metal-organic framework;
Nanozyme
- From:
Chinese Journal of Analytical Chemistry
2025;53(6):924-933,中插6-中插15
- CountryChina
- Language:Chinese
-
Abstract:
Glutathione(GSH)is a tripeptide containing sulfhydryl groups,which has the function of antioxidant,detoxifying,and immune enhancing effects,and its expression is closely associated with many diseases such as cancer,etc.Therefore,it is of great significance for clinical diagnosis by developing a highly sensitive,simple,and rapid method for detecting GSH.Herein,two-dimensional(2D)Zn-TCPP(Fe)nanosheets employing Zn2+and Fe-bound tetrakis(4-carboxyphenyl)porphyrin ligands were obtained by a surfactant-assisted synthetic method.The 2D Zn-TCPP(Fe)nanosheets exhibited excellent peroxidase-like activity,which allowed the catalysis of the H2O2-initiated oxidation of colorless 3,3',5,5'-tetramethylbenzidine(TMB)to blue oxidized TMB(ox-TMB).In the presence of GSH,GSH underwent a reduction reaction with oxTMB,resulting in the blue color of the solution fading.The proposed colorimetric sensor exhibited favorable sensitivity for GSH in the linear range of 0.1-200 μmol/L with a limit of detection(S/N=3)of 0.042 μmol/L,indicating excellent selectivity.The developed sensor was successfully applied to detect GSH level in the human serum samples with recoveries of 93.0％-107.7％,showing satisfactory results.The developed colorimetric sensor provided a new approach for detecting GSH.
