An RNA-cleaving DNAzyme and G-Quadruplex DNAzyme-Cascade System for Sensing of Small Molecules in Organic Cosolvent
10.19756/j.issn.0253-3820.251062
- VernacularTitle:在有机溶剂体系中构建由RNA切割型脱氧核酶和G-四链体脱氧核酶串联的小分子传感系统
- Author:
Su-Hui LI
1
;
Shuai-Qi ZHANG
;
Yi-Bo ZHANG
;
Quan ZHENG
;
Tian-Jun CHANG
Author Information
1. 河南理工大学资源环境学院,焦作 454000
- Keywords:
DNAzyme;
Aptazyme;
Organic solvent;
Biosensors;
Visualization
- From:
Chinese Journal of Analytical Chemistry
2025;53(6):914-923
- CountryChina
- Language:Chinese
-
Abstract:
Detection of poor-water-soluble substances typically requires an organic solvent containing solution,and therefore,the biosensors that can operate in such conditions are highly promising for practical applications.However,most existing biosensors have been developed in aqueous solution and it is hard to work effectively in organic solvent systems.In this work,we uncovered that the G-quadruplex(G4)/hemin DNAzyme(G4HD)could be strongly reactivated by NH4 in organic solvents at concentration up to 30％,and the activity was significantly higher than that in aqueous solutions containing K+.Based on this discovery,a biosensing system was developed for small molecule in 40％methanol by coupling G4HD with our previously identified RNA-cleaving DNAzyme(E3).This system comprised an ATP-activated aptazyme that was made of E3 and a G4HD that was activated by the cleavage product of the aptazyme.By using a manmade pipette tip filter to separate the reaction products,the subsequent colorimetric reaction of the G4HD could be triggered.This biosensing system enabled the visualization of target molecules in organic cosolvents without any other instruments.The activable DNAzyme-cascade sensing system had potential in point-of-care detection of poor-water-soluble pollutants,thereby enhancing the practical values of functional nucleic acid-based biosensors in real-world detection conditions.
