Synthesis of A New Naphthalenesulfonamide-based"Turn-on"Fluorescent Probe for Rapid Detection of Glyphosate
10.19756/j.issn.0253-3820.241477
- VernacularTitle:新型萘磺酰胺类开启型荧光探针的合成及用于草甘膦快速检测
- Author:
Rong-Rong ZHAO
1
;
Hong-Lin LIU
;
Ying-Ping HUANG
;
Cui-Wen DENG
;
Song-Yan LI
;
Shui-Lian YU
;
Mao-Sheng TAO
;
Yi-Qun TIAN
;
Xi YUAN
Author Information
1. 三峡大学水利与环境学院,宜昌 443002;三峡大学,三峡库区生态环境教育部工程研究中心,宜昌 443002
- Keywords:
Naphthalenesulfonamide-based fluorescent probes;
Glyphosate;
Photoinduced electron transfer;
Sensitivity;
Selectivity
- From:
Chinese Journal of Analytical Chemistry
2025;53(6):903-913
- CountryChina
- Language:Chinese
-
Abstract:
Widespread utilization of glyphosate has led to environmental residues,posing potential threats to ecological systems and human health.Traditional methods for detection of glyphosate are limited by specialized equipment and operational techniques,resulting in inefficient responses.Therefore,it is urgent to develop a convenient,sensitive and accurate detection method for detection of glyphosate.Herein,a new naphthalenesulfonamide-based"Turn-on"fluorescent probe was synthesized using 2-chloroaniline and dansyl chloride as raw materials through a one-step process,which showed a good linear relationship between the glyphosate concentration in concentration range of 0.003-70 μmol/L and the fluorescence intensity(R2=0.995),with a detection limit of 2.73 nmol/L(S/N=3).Analytical techniques such as nuclear magnetic resonance(NMR)spectroscopy and high-resolution mass spectrometry(HRMS)were used to investigate the interaction mechanism between the fluorescent probe and glyphosate.The results indicated that a nucleophilic substitution reaction occurred between the probe and the secondary amine(—NH—)of glyphosate,inducing a photoinduced electron transfer(PET)effect which enhanced the fluorescence intensity by 11.2 times.The probe showed good anti-interference ability towards coexisting metal ions,anions and pesticides in water.When applied to determination of glyphosate in the samples such as tap water,river water(Xiangxi River Reservoir),soil,soybeans,and corn,the spiking recoveries ranged from 94.7％to 109.9％,demonstrating the high accuracy and broad applicability of this detection method.A portable test strip based on this fluorescent probe was developed for rapid semi-quantitative analysis of glyphosate.The developed method was rapid,sensitive,and portable,providing theoretical and technical support for on-site measurement of environmental contaminants.
