Study on Colorimetric Sensor Array Based on Enzymatic Method for Highly Selective Detection of Sarin
10.19756/j.issn.0253-3820.251027
- VernacularTitle:基于酶法高选择性检测沙林的比色阵列传感器研究
- Author:
Lian-Bo JIANG
1
;
Guo-Hong LIU
;
Zhuang-Hu XU
;
Jian LI
;
Yong-Ling SHEN
;
Cai-Xia XU
;
Chuan-Qin ZANG
;
Yan-Hua XIAO
;
Dan-Ping LI
;
Ting LIANG
Author Information
1. 陆军防化学院,北京 102205
- Keywords:
Colorimetric sensor array;
Sarin;
Enzymatic method;
Field detection
- From:
Chinese Journal of Analytical Chemistry
2025;53(5):832-841,中插21-中插23
- CountryChina
- Language:Chinese
-
Abstract:
Sarin(GB)is a typical representative of nerve agents with high toxicity,and very low amount can cause death.GB can cause water and atmospheric environment poisoning,so the detection of GB in water and air is of great significance.In this work,a colorimetric sensor array(CSA)based on GB inhibition of cholinesterase activity was constructed to detect GB with high selectivity.A 4×4 colorimetric array was constructed using acetylcholinesterase(AChE),butyryl cholinesterase(BuChE)and the corresponding substrate acetylthiocholine iodide(S-ACh),butyryl thiocholine iodide(S-BCh),acetylcholine chloride(ACh),butyryl choline chloride(BCh)and 2,6-dichloroindophenol ethyl ester(DCIE).The linear curve of the sensor was Y=131.3×lgC+271.6(R2=0.997),where Y was the array response Euclidean distance,C was the concentration of GB(mg/L),the linear range was 0.03?0.32 mg/L,and the detection limit was 27.6 μg/L.The method could effectively distinguish chemical warfare agents(CWA)such as VX,Soman(GD),mustard gas(HD),Louie reagent(L),and had high anti-interference ability,sensitivity and good repeatability.It was successfully applied to the detection of GB in simulated water and simulated air samples,and the sample recovery rate was 97.2% ?100.9%.This method would be potentially applied to the field rapid detection of nerve agents.
