Reverse Fluorescence Enhancement Test Strip for Detection of Interleukin-8 Biomarker in Oral Squamous Cell Carcinoma
10.19756/j.issn.0253-3820.241205
- VernacularTitle:反向荧光增强试纸条检测口腔鳞状细胞癌标志物白细胞介素8
- Author:
Qiu-Ting ZHANG
1
;
Yi XU
;
Shuang ZHAO
;
Xiao-Qun GONG
Author Information
1. 天津大学生命科学学院,天津 300072
- Keywords:
Reverse fluorescence-enhanced test strips;
Oral squamous cell carcinoma;
Interleukin-8
- From:
Chinese Journal of Analytical Chemistry
2025;53(5):823-831
- CountryChina
- Language:Chinese
-
Abstract:
Oral squamous cell carcinoma(OSCC)is a common malignant tumor.Interleukin-8(IL-8)is an important biomarker of OSCC,and its level can reflect the occurrence and development of OSCC.It is of great significance to detect IL-8 rapidly and sensitively for the purpose of early diagnosis of OSCC.In this study,gold nanoparticles(AuNPs)with uniform particle size were synthesized by reduction of chloroauric acid with trisodium citrate,and the probe(AuNPs@mAb1)was prepared by coupling AuNPs with the murine anti-IL-8 monoclonal antibody mAb1.Cy5-NHS and murine anti-IL-8 monoclonal antibody mAb2 complex(Cy5-mAb2)and sheep anti-mouse IgG antibody were sprayed on nitrocellulose membrane to form test line(T line)and control line(C line)respectively,and reverse fluorescence-enhanced test strips were thus constructed,based on which an immunochromatographic method was established for highly sensitive detection of IL-8 in saliva samples.The experimental results showed that the test strip had good stability,high specificity and high sensitivity.The linear range for fluorescence detection of IL-8 was 0.01-100 ng/mL,and the limit of detection(3σ)was 7.93 pg/mL.The linear range for visualization detection was 6-100 ng/mL,with limit of detection(3σ)of 0.85 ng/mL.The fabricated test trip had good preparation reproducibility,with inter-and intra-batch assay precision of less than 5.5%.The test strip was used for detection of IL-8 in healthy human saliva samples,with spiked recoveries of 93.7% -102.4%,and relative standard deviations of 2.1% -4.3%.The fabricated test strip could be used for early screening of OSCC.
