Competitive Immunoassay for Detection of Enrofloxacin Based on Metasurface Plasma Resonance Chip Coupled with Gold Nanoparticles
10.19756/j.issn.0253-3820.231377
- VernacularTitle:纳米超表面等离子体共振芯片耦合金纳米颗粒的免疫竞争法检测恩诺沙星
- Author:
Wei-Hao JI
1
;
Hong-Li FAN
;
Lei GONG
;
Li-Ping HUANG
;
Xiao-Long FAN
;
Jia-Yong HU
;
Tao-Hong ZHOU
;
Gang LIU
Author Information
1. 湖北省食品质量安全监督检验研究院,武汉 430075;国家市场监管重点实验室(动物源性食品中重点化学危害物检测技术),武汉 430075;湖北时珍实验室,武汉 430065
- Keywords:
Meta-surface plasma resonance;
Biochip;
Gold nanoparticles;
Competitive immunoassay;
Enrofloxacin
- From:
Chinese Journal of Analytical Chemistry
2025;53(5):814-822
- CountryChina
- Language:Chinese
-
Abstract:
Risks of food safety induced by small molecule drug residues in animal food and environment have become an increasing public concern,so it is necessary to develop highly sensitive and easy-to-operate techniques to detect small molecules.Herein,a metasurface plasma resonance(MetaSPR)sensor chip coupled with gold nanoparticles(AuNPs)was developed for detection of enrofloxacin(ENR)based on competitive immunoassay.The detection range of the sensor for ENR was 0.025-3.2 ng/mL,and the detection limit(3σ)was 20 pg/mL.The biosensor showed excellent performance including high selectivity,good stability,ease to operate and high throughput,etc.The developed method was applied to detection of ENR residues in real samples,with recoveies of 96.0% -105.0%.The proposed sensing strategy provided new technique reference for detection of other small molecules in the field of residue analysis in food safety and environment monitoring.
