Rapid Detection of p53 Gene Based on Rolling Circle Amplification and Berberine Hydrochloride
10.19756/j.issn.0253-3820.241368
- VernacularTitle:基于滚环扩增及盐酸小檗碱快速检测p53基因
- Author:
Jing-Yan ZHANG
1
;
Yu-Ping ZHANG
;
Lin-Hui XIE
;
Hong ZHOU
;
Si-Yao LUO
;
Ying-Ping LUO
Author Information
1. 川北医学院药学院,南充 637000
- Keywords:
p53 gene;
Rolling circle amplification;
Label-free;
Berberine hydrochloride;
Fluorescence detection
- From:
Chinese Journal of Analytical Chemistry
2025;53(5):785-793
- CountryChina
- Language:Chinese
-
Abstract:
In this work,a rapid and label-free sensing platform was designed for visual detection of p53 gene.The rolling circle amplification(RCA)process of the assay platform was activated by p53 gene to produce long DNA-wires,which could bound with berberine hydrochloride(BBH)and further enhanced its fluorescence.This method showed high sensitivity with a low detection limit of 5.63 pmol/L,and high specificity toward p53 gene over other interference materials,even for single-base mutation gene.The method could realize the visual detection of targets under the illumination of a UV lamp.In addition,the designed fluorescence detection platform was successfully applied to p53 gene analysis in 10% fetal bovine serum samples,and the relative standard deviation and the recoveries were 0.1% -1.2% and 99.5% -104.7%,respectively.This approach had satisfactory characteristics,such as low cost,label-free,rapidity,high sensitivity,good selectivity and anti-interference ability,and reliable detection capability for complex practical samples,demonstrating a promising prospect in the diagnosis and treatment of diseases,especially for cancer.
