Research on In-Situ Extractive Ionization for Original Ecological Samples and Its Miniature Device
10.19756/j.issn.0253-3820.241387
- VernacularTitle:原生态样品原位萃取离子化技术及小型装置研究
- Author:
Xiao-Feng DONG
1
;
Feng LIU
;
You-Han XUE
;
Xi-De YE
;
Shuang-Long WANG
Author Information
1. 东华理工大学机械与电子工程学院,南昌 330013
- Keywords:
Ambient mass spectrometry;
In-situ extractive ionization;
Miniature device;
Traditional Chinese medicine detection
- From:
Chinese Journal of Analytical Chemistry
2025;53(5):749-757
- CountryChina
- Language:Chinese
-
Abstract:
Current ambient mass spectrometry ionization often requires external auxiliary equipment such as high-voltage power supply,gas cylinder,and syringe pump.Moreover,the process of sample preparation is cumbersome,and the experimental operations are complex,which makes it difficult to adapt to real-time on-site detection.In this work,a novel method was proposed,in which direct sampling of raw samples,online extraction of interest analytes,and ionization of target molecules were integrated into a single unit.With the developed method,the in-situ extraction and nano-electrospray ionization for both liquid and solid raw samples were achieved.Also,a handheld ion source and its pose adjustment device were developed,and the position and angle parameters were subsequently optimized.The performance of the ionization device was tested using standard solutions of caffeine and reserpine.The limits of detection(LODs)were 0.08 μg/L and 0.14 μg/L,with relative standard deviations(RSDs)≤3.7% and≤5.6%,respectively,indicating that the device possessed high sensitivity and stability.Using this device,three different concentrations of reserpine standard solutions were continuously tested for five days.The intra-day RSDs were consistently≤4.7% and the inter-day RSDs were all≤10.3%,showing the good working stability of the device.Without any pretreatment,a rapid qualitative detection of medicinal components including astragaloside II and cycloastragenol in five traditional Chinese medicines was carried out,with RSDs≤8.0% and≤7.1%,respectively.Additionally,rapid qualitative detection of gallic acid,a medicinal component,in white peony roots,and hypaphorine as well as quercetin in cowherb seeds were carried out,with RSDs≤7.0%,≤6.4% and≤6.1%,respectively.These results demonstrated that the ionization technology and device exhibited good stability during qualitative detection of raw samples.
