Construction of Artificial Cells with the Ability to Secrete and Receive Quorum Sensing Signals and Study on Their Signal Transduction
10.19756/j.issn.0253-3820.251081
- VernacularTitle:具备群体感应信号分泌和接收能力的人造细胞的构建及其信号转导研究
- Author:
Hang DU
1
;
Jing-Jing ZHAO
;
Shu-Bin LI
;
Xiao-Jun HAN
Author Information
1. 哈尔滨工业大学化工与化学学院,城市水资源与水环境国家重点实验室,哈尔滨 150001
- Keywords:
Escherichia coli;
Quorum sensing;
Signal molecules;
Artificial cells
- From:
Chinese Journal of Analytical Chemistry
2025;53(5):681-688
- CountryChina
- Language:Chinese
-
Abstract:
The bottom-up construction of artificial cells to mimic signal transduction is of great significance for a deep understanding of the communication networks of complex living systems.The dynamic coupling of signal secretion and response among artificial cells poses an important challenge.In this work,two types of artificial cells were constructed through a bio-artificial hybrid strategy.One was an artificial cell as an AI-2 signal sender(sender),which contained bacteria capable of autonomously synthesizing AI-2 signaling molecules.The other was an artificial cell as an AI-2 signal receiver(receiver),which contained bacteria expressing green fluorescent protein in response to the corresponding AI-2 signal.When the ratio of senders to receivers was 4∶1,the fluorescent signal of the receivers gradually increased over time and reached a plateau at 240 minutes.The artificial cells capable of sending and receiving quorum sensing signaling molecules constructed in this work laid a foundation for the study of complex signal communication within artificial cell populations.
