Preparation and In Vitro Degradation Characteristics Analysis of Poly(lactic-co-glycolide)Microspheres Based on Microfluidic Process
10.19756/j.issn.0253-3820.251038
- VernacularTitle:基于微流控工艺制备丙交酯乙交酯共聚物微球及其降解和释药特性分析
- Author:
Bao-Cheng WANG
1
;
Cong-Yu MA
;
Ke WANG
;
Si-Tong ZHENG
;
Xiao-Yan ZHANG
;
Yue-Mei ZHAO
;
Xun ZHAO
;
Jian-Bin PAN
;
Zheng-Song GAO
;
Hai-Wei SHI
;
Yao-Zuo YUAN
;
Hong-Yuan CHEN
Author Information
1. 南京威尔药业集团股份有限公司,南京 210042
- Keywords:
Poly(lactide-co-glycolide);
Microfluidic;
Microsphere;
Environmental factor;
Degradation characteristic
- From:
Chinese Journal of Analytical Chemistry
2025;53(4):621-630
- CountryChina
- Language:Chinese
-
Abstract:
Poly(lactic-co-glycolide)(PLGA)is a key excipient in long-acting sustained-release preparations,and its degradation properties directly affect the drug release behavior.In this study,PLGA microspheres were prepared by microfluidic techniques,and the morphology changes of the microspheres were observed by scanning electron microscopy(SEM).In alkaline environment,due to the accelerated hydrolysis of ester bonds,the surface of the microspheres was rapidly dissolved and eroded,and the degradation rate was significantly higher than that in acidic environment.High temperature accelerated the degradation of PLGA microspheres.Under neutral and alkaline conditions,the microspheres showed aggregation and adhesion.Under acidic conditions,the microspheres gradually decomposed into irregular fragments.The high ionic strength further promoted the surface corrosion of the microspheres,especially under extreme pH conditions.Simultaneously,PLGA microspheres encapsulating coumarin were prepared to simulate the microsphere formulation.The release rate of coumarin after degradation of the microspheres under different conditions was observed by measuring the absorbance with ultraviolet-visible spectrophotometry.The results were consistent with those of the blank microspheres.This study revealed that the degradation of PLGA microspheres was significantly pH-dependent,temperature sensitive and ion strength responsive.These findings not only helped to understand and optimize the long-term stability and controlled release performance of drug-carrying microspheres,but also provided a theoretical basis for further improvement of PLGA-based drug carrier design.
