Study on Regulation of Liquid-Liquid Phase Separation of Ribonucleic Acid with CAG Repeats
10.19756/j.issn.0253-3820.241337
- VernacularTitle:CAG重复序列核糖核酸液-液相分离的调控研究
- Author:
Ying-Xue MA
1
;
Zhou GONG
;
Zi-Han LI
;
Chun TANG
Author Information
1. 中国科学院武汉文献情报中心,科技大数据湖北省重点实验室,武汉 430071;中国科学院大学经济与管理学院信息资源管理系,武汉 430071;中国科学院精密测量科学与技术创新研究院,武汉 430071
- Keywords:
Ribonucleic acid with CAG repeats;
Liquid-liquid phase separation;
Raman spectroscopy;
Phase separation regulation
- From:
Chinese Journal of Analytical Chemistry
2025;53(4):612-620
- CountryChina
- Language:Chinese
-
Abstract:
Ribonucleic acid(RNA)molecules with repeat expansion sequences can liquid-liquid phase separate into droplet-like condensates,which usually have good fluidity and can dynamically exchange matter and energy with the outside world,so as to play physiological functions.However,when the conditions are changed,the droplets will undergo a phase transition,forming gel-like or solid-like condensates.The change in the morphology of the condensates is closely related to many diseases.In this work,the effects of the RNA repeat length,concentration,cation and pH value on the liquid-liquid phase separation(LLPS)of RNA with CAG repeats were systematically studied,and the molecular structure of the condensates formed by LLPS was analyzed by Raman spectroscopy.This study revealed the molecular basis of the formation of different condensates from the perspective of structural biology,and provided new insights for in-depth understanding of the molecular assembly of RNA phase separation and phase transition.
