Nitrogen-doped Carbon-supported Ruthenium Metal Atom Nanozyme for Colorimetric Determination of Xanthine Oxidase Inhibitors
10.19756/j.issn.0253-3820.231402
- VernacularTitle:氮掺杂碳载钌金属原子纳米酶用于黄嘌呤氧化酶活性分析及其抑制剂的比色检测
- Author:
Quan-Yi LIU
1
;
Qi FANG
;
Hui WEI
;
Yan DU
Author Information
1. 中国科学院长春应用化学研究所,电分析化学国家重点实验室,长春 130022;中国科学技术大学应用化学与工程学院,合肥 230026
- Keywords:
Nanozyme;
Colorimetric analysis;
Hydrogen peroxide;
Xanthine oxidase;
Xanthine oxidase inhibitor
- From:
Chinese Journal of Analytical Chemistry
2025;53(4):516-524
- CountryChina
- Language:Chinese
-
Abstract:
A colorimetric detection method for xanthine oxidase activity and its inhibitors(XOI)based on nitrogen-doped carbon-supported ruthenium metal atom(Ru/NC)nanozyme was developed.Through a host-guest strategy,Ru/NC nanozyme with excellent peroxidase-like activity was prepared using zeolitic imidazolate framework-8(ZIF-8)as the host molecule and ruthenium acetylacetonate(Ru(acac)3)as the guest molecule.Based on its peroxidase-like catalytic activity,the visual method for detection of hydrogen peroxide(H 2O 2)and analysis of xanthine oxidase(XO)activity were developed.Under optimized experimental conditions,the linear detection range for H 2O 2 was 10-500 μmol/L,with a detection limit of 4.8 μmol/L(3σ);for XO activity analysis,the linear range was 1-7 mU/mL,with a detection limit of 0.69 mU/mL(3σ).Furthermore,an effective method for screening XOI was developed,which had the advantages of low cost,simplicity,and visualization.
