Fast Screening of 10 Kinds of Illegal Aphrodisiac Drugs in Foods by Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
10.19756/j.issn.0253-3820.221020
- VernacularTitle:基质辅助激光解吸电离-飞行时间质谱法快速筛查食品中10种非法壮阳抗疲劳类药物
- Author:
Xi ZENG
1
;
Yu WANG
;
Ying-Li CHEN
;
Tian-Tian ZHAO
;
Zhen-Lin XU
;
Heng-Xin ZHOU
Author Information
1. 广州市食品检验所,广州 511400;华南农业大学食品学院,广东省食品质量安全重点实验室,广州 510642
- Keywords:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;
Aphrodisiac drugs;
Illegal addition;
α-Cyano-4-hydroxycinnamic acid;
Sinapic acid
- From:
Chinese Journal of Analytical Chemistry
2025;53(3):441-450,中插3-中插5
- CountryChina
- Language:Chinese
-
Abstract:
A matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)method was developed for simultaneous analysis of 10 kinds of aphrodisiac drugs,including sildenafil,acetildenafil,nor-acetildenafil,homosildenafil,lodenafil carbonate,sildlenafil dimer impurity,vardenafil dimer,hydroxyacetildenafil,N-desmethylsildenafil and udenafil.By using different matrices(α-cyano-4-hydroxycinnamic acid(HCCA)and sinapic acid(SA)),the effects of solvents,amount of matrices,laser intensity and spotting methods on the peak strength and signal intensity of MALDI-TOF MS for the aphrodisiac drugs were investigated.As a result,SA was chosen as the matrix,and then dispersed in methanol-water(50∶50,V/V),and spotted by matrix firstly and sample secondly approach with the reflect linear positive mode and laser power of 60%.Under optimal conditions,the proposed MALDI-TOF MS method could obtain stable signal,high intensity and well-repeated mass spectrometric results.The results of method validation showed a linear range from 10 to 100 ng/mL,and the regression coefficients(r)were all above 0.985.The limits of detection(LOD)were 0.1-1.0 ng/mL,and the recoveries were in the range of 72.9%-109.9%for health wine,soft capsule and instant coffee samples,while the relative standard deviations(RSDs)ranged from 0.7%to 11.3%(n=3).The test results of 10 kinds of aphrodisiac drugs by MALDI-TOF MS were in accordance with the results of high-performance liquid chromatography-tandem mass spectrometry.This method exhibited high sensitivity,high accuracy,good anti-interference ability,low chemical solvents consumption and environmentally friendliness,and could be used to detect drugs without standards.The developed MALDI-TOF MS method could realize the non-target screening and detection of 10 kinds of illegally added aphrodisiac drugs in foods.
