Microchannel-based Electrochemiluminescence Sensor for Tetracycline Detection Using Luminol/Hydrogen Peroxide as Reporter System
10.19756/j.issn.0253-3820.251013
- VernacularTitle:以鲁米诺/过氧化氢作为报告体系的微通道电化学发光传感器用于四环素的检测
- Author:
Shao-Kun HUANG
1
;
Xiu-Lin XIE
;
Hua-Bin CAI
;
Yan-Ling HUANG
;
Yue LIN
;
Zhen-Yu LIN
Author Information
1. 福州大学化学学院,食品安全与生物分析教育部重点实验室,福建省食品安全分析与检测重点实验室,福州 350116
- Keywords:
Electrochemiluminescence;
Luminol;
Microchannel;
Tetracycline;
Aptamer
- From:
Chinese Journal of Analytical Chemistry
2025;53(3):356-363
- CountryChina
- Language:Chinese
-
Abstract:
A microchannel-based electrochemiluminescence(ECL)sensor was developed for detection of tetracycline(TC)utilizing luminol/H2O2 as ECL reporting system.The low excitation potential of luminol/H2O2 effectively mitigated the impact of clamping voltage,thereby enhancing the detection performance of the microchannel-based ECL sensor.The microchannel modified with TC aptamer selectively recognized and captured target TC.The positively charged TC reduced the surface charge density within the microchannel,thereby increasing the ionic current in the microchannel,leading to change of ECL signal of system.The experimental conditions such as electrolyte concentration,TC-aptamer concentration,and reaction time between TC and TC-aptamer were optimized.Under optimal conditions,the difference of ECL signal in the absence and presence of TC(?ECL)exhibited a good linear relationship with TC concentration in the range from 1.00 ng/mL to 200 ng/mL,with a detection limit as low as 0.69 ng/mL.The sensor had good selectivity and was successfully used in detection of TC in milk samples.
