Colorimetric Detection of Ascorbic Acid Based on Oxidase-like Activity of Fe2O3/Nitrogen-doped Carbon Nanomaterials
10.19756/j.issn.0253-3820.251029
- VernacularTitle:基于Fe2O3/氮掺杂碳纳米材料类氧化酶活性的抗坏血酸比色检测
- Author:
Huan ZHOU
1
;
Hao LUO
;
Yu TONG
;
Qian-Fen ZHUANG
;
Yong WANG
Author Information
1. 南昌大学化学化工学院,南昌 330031
- Keywords:
Ascorbic acid;
Oxidase mimic;
Colorimetric sensor;
Nanozyme
- From:
Chinese Journal of Analytical Chemistry
2025;53(3):346-355
- CountryChina
- Language:Chinese
-
Abstract:
Ferric oxide/nitrogen-doped carbon nanomaterials(Fe2O3/N-C)with high oxidase-like activity were successfully synthesized via the wet chemistry and pyrolysis method using pyrrole and 1,2,3,4-butanetetracarboxylic acid as raw materials and ferric chloride as the oxidant.The structure and morphology of Fe2O3/N-C were characterized by the techniques including scanning electron microscopy,surface scanning elemental analysis,X-ray diffraction,X-ray photoelectron spectroscopy,and Fourier transform infrared spectroscopy.It was revealed that Fe2O3/N-C could efficiently catalyze the conversion of colorless 3,3',5,5'-tetramethylbenzidine(TMB)into blue-colored oxidized TMB(oxTMB).Based on the principle that ascorbic acid(AA)could inhibit the catalytic color-development reaction of Fe2O3/N-C on TMB,resulting in a paler color and a reduction in the absorbance of the system,a colorimetric sensor for sensitive and accurate detection of AA was constructed.The linear range of the sensor for AA detection was 0.25-30.0 μmol/L,and the detection limit was 0.1 μmol/L.Moreover,it was successfully applied to determination of AA in beverage and tablet samples with satisfactory results.
