Construction of A Single-cell Metabolomics Mass Spectrometry Analysis Platform Enabling Continuous Injection Based on Ultrasound
10.19756/j.issn.0253-3820.241441
- VernacularTitle:基于超声可连续进样的完整单细胞代谢组学质谱分析平台的构建
- Author:
Wen-Mei ZHANG
1
;
Xiao-Kai GUO
;
Tai-Lin XU
;
Guang-Sheng GUO
;
Xia-Yan WANG
Author Information
1. 北京工业大学化学与生命科学学院化学系,环境安全与生物效应卓越中心,北京市绿色催化与分离重点实验室,北京 100124
- Keywords:
Single-cell;
Mass spectrometry;
Ultrasound;
Narrow bore capillary;
Metabolite analysis
- From:
Chinese Journal of Analytical Chemistry
2025;53(3):338-345
- CountryChina
- Language:Chinese
-
Abstract:
Single-cell metabolite analysis at the small molecule level reveals intercellular heterogeneity and molecular diversity,especially living cell metabolite analysis which can provide more accurate biochemical information.In this study,a comprehensive single-cell metabolomics mass spectrometry analysis platform was constructed based on continuous ultrasonic sample introduction,aiming to improve the utilization rate of single cells and the efficiency of mass spectrometry detection.This platform utilized mechanical motion generated by a miniaturized ultrasound module,which minimally affected cell integrity and viability,enabling cell suspension and dispersion for up to 60 min,with cell viability exceeding 70%.By comparing cell suspension densities and the cell number of mass spectrometry detections between static and ultrasound groups,the results showed that the ultrasound treatment significantly reduced cell sedimentation rate and increased single-cell mass spectrometry detection efficiency.Applying this platform to single-cell analysis of cell line of mouse cerebellar astrocytes(C8D1A)and mouse glioma(GL261)cells achieved clustering and differential analysis of different cell types,demonstrating the method's potential in analyzing cellular heterogeneity and identifying cells.This approach promised to provide new insights and solutions for single-cell analysis.
