Fluorescence Method for Determination of Metolazone and Valsartan in Human Serum and Urine:A Comparison from Zero-order to Second-order Calibration Method
10.19756/j.issn.0253-3820.231132
- VernacularTitle:荧光法测定人血液和尿液中的美托拉宗和缬沙坦:从零阶校正到二阶校正的比较
- Author:
Zi-Wei DING
1
;
Hai-Long WU
;
Xiao-Zhi WANG
;
Tong WANG
;
Hao-Ran LIU
Author Information
1. 湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,长沙 410082
- Keywords:
Chemometrics;
Alternating normalization-weighted error;
Excitation-emission matrix fluorescence spectroscopy;
Metolazone;
Valsartan
- From:
Chinese Journal of Analytical Chemistry
2025;53(2):224-234
- CountryChina
- Language:Chinese
-
Abstract:
A second-order calibration method combined with excitation-emission matrix(EEM)fluorescence spectroscopy was presented for simultaneous quantitative analysis of two anti-hypertensive drugs,metolazone(MET)and valsartan(VAL),in human serum and urine,and the quantitative results were compared with the results obtained by zero-and first-order calibration methods.The results indicated that the methods based on zero-and first-order calibration were inadequate for accurately quantifying the components of interest in cases where severe spectral overlap and unknown interferences coexisted.However,it was possible to obtain satisfactory results with the second-order calibration method based on alternating normalization-weighted error(ANWE)algorithm because of its strong"mathematical separation",even when the fluorescence spectra of the target analytes and unknown interferents considerably overlapped.Correlation coefficients for both analytes were greater than 0.99,with mean recoveries of 104.9%±5.7%and 107.8%±9.2%for MET and VAL in human serum,and 103.7%±8.9%and 94.7%±3.8%in human urine,respectively.In addition,the sensitivity,selectivity,limit of detection,limit of quantification,repeatability,and reproducibility of the proposed second-order calibration method were thoroughly examined.All results indicated that the established method was capable of achieving simultaneous and accurate quantification of MET and VAL in human body fluids,which was expected to be applied to analysis of both drugs in clinical settings.
