Prefabricated Nano Carbon-Platinum Composite Matrix for Surface-assisted Laser Desorption Ionization Mass Spectrometry Imaging of Small Molecular Components in Plant Tissues
10.19756/j.issn.0253-3820.241296
- VernacularTitle:预制纳米碳-铂复合基质用于表面辅助激光解吸电离质谱成像分析植物组织中的小分子成分
- Author:
Fan YANG
1
;
Yu-Lin SHEN
;
Can GONG
;
Zhao-Xin LIU
;
Qiang-Sheng GUO
;
Xu XU
Author Information
1. 上海应用技术大学化学与环境工程学院,上海 201418
- Keywords:
Surface assisted laser desorption ionization mass spectrometry;
Mass spectrometry imaging;
Prefabricated matrix;
Carbon-platinum composite nanomaterials
- From:
Chinese Journal of Analytical Chemistry
2025;53(2):204-213,中插1-中插2
- CountryChina
- Language:Chinese
-
Abstract:
Compared with matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS)using organic small molecule matrix,surface-assisted laser desorption ionization mass spectrometry(SALDI-MS)based on nanomaterial matrix is more effective in analysis of small molecule compounds.Ion sputtering instruments have obvious advantages for applying inorganic nanomatrix.In this work,the carbon-platinum material was sputtered onto a glass cover slip using an ion sputtering instrument to form a carbon-platinum(C-Pt)composite nanomatrix,and an SALDI-MS analytical method was thus established based on the prefabricated C-Pt composite matrix.The experimental results showed that the C-Pt composite nanomatrix could significantly improve the signal intensity and signal-to-noise ratio of the mass spectrum peaks of the components to be measured.The ratio of carbon to platinum,the duration of ion sputtering,and the laser power in mass spectrometer were investigated to select the optimal C-Pt matrix prefabricated conditions and SALDI-MS experimental conditions.Using the prefabricated C-Pt composite matrix,the melittriose and daidzein sample solution were applied as sample to examine repeatability.The results showed that the intra-point repeatability(RSD)was≤4.8%and the inter-point repeatability(RSD)was≤6.4%.The quercetin and melitriose were applied as model samples,and a linearity between MS peak intensity and respective concentration in the range of 0.05-1.0 mg/mL was found,with linear correlation coefficients(R2)greater than 0.994,showing good potential for quantitative and imaging analysis.Then,the prefabricated C-Pt composite matrix was applied to SALDI-MS analysis of the 50%ethanol extract of soybean,and 15 kinds of compounds including oligosaccharides and triglycerides were identified from the mass spectra.Furthermore,the C-Pt matrix was employed in SALDI-MS imaging for the compositions in corn tissue section,and the results showed that diglycerides and triglycerides were mainly distributed in the corn embryo and around the embryo,and the distribution of oligosaccharides was relatively even.
