Laccase-like Nanozyme Prepared with Coordination Strategy and Their Analytical Applications
10.19756/j.issn.0253-3820.241481
- VernacularTitle:配位策略制备的类漆酶纳米酶及其分析应用
- Author:
Bin-Fu WANG
1
;
Zi-Ruo ZHANG
;
Qi GAO
;
Hao-Di XU
;
Wen-Ying LI
;
Ding-Yi TONG
Author Information
1. 宁波大学材料科学与化学工程学院,宁波 315201
- Keywords:
Laccase;
Laccase-like nanozyme;
Coordination strategy;
Analytical applications;
Review
- From:
Chinese Journal of Analytical Chemistry
2025;53(2):164-175
- CountryChina
- Language:Chinese
-
Abstract:
Laccase is a type of polyphenol oxidase that can catalyze the oxidation of various substances,including phenols,aromatic amines,and catecholamines.It has been widely utilized in pollutant degradation and analytical applications.However,the high cost of preparation of natural laccase and its susceptibility to environmental factors,which can lead to denaturation and inactivation,limit its practical applications.Nanozymes,which are nanomaterials that exhibit enzyme-like properties,offer advantages such as easy preparation,adjustable activity,and exceptional stability.Currently,many types of nanozymes have been developed.Inspired by the coordination of Cu2+with amino acids in the active site of natural laccase,researchers have employed coordination synthetic strategies to prepare laccase-like nanozymes.The metal nodes in these laccase-like nanozymes include copper,manganese,and cerium,while the ligands involve a variety of chemicals like nucleotides,amino acids,polypeptides,and aromatic acids.By manipulating factors such as the metal-to-ligand ratio,reducing capacity of ligands,buffer solutions,chloride ions,bromine ions,the catalytic activity of laccase-like nanozymes can be finely tuned.In this paper,laccase-like nanozymes developed through coordination strategies were categorized and summarized,along with review of their analytical applications in detection of phenolic compounds,disease biomarkers,antibiotics,pesticides,sulfur-containing pollutants,and time-temperature indicators.Furthermore,the challenges currently faced in the research of laccase-like nanozymes and future research directions were discussed.
