Identification of Salmonella Using High Performance Liquid Chromatography-Tandem Mass Spectrometry Based on Characteristics Peptides
10.19756/j.issn.0253-3820.241114
- VernacularTitle:基于特征肽的高效液相色谱-串联质谱法鉴定沙门氏菌
- Author:
Meng-Qi CHEN
1
;
Mu-Yun YUAN
;
Cheng-Dong HUANG
;
Jing-Wen LIU
;
Guang-Feng ZENG
;
Wen-Rui CHEN
;
Song-Qing HU
;
Li LING
Author Information
1. 广州海关技术中心,广州 510623;华南理工大学食品科学与工程学院,广州 510641
- Keywords:
Salmonella;
Peptide biomarkers;
High performance liquid chromatography-tandem mass spectrometry;
Multiple reaction monitoring
- From:
Chinese Journal of Analytical Chemistry
2024;52(12):1913-1922
- CountryChina
- Language:Chinese
-
Abstract:
A qualitative identification method for Salmonella using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)with multiple reaction monitoring(MRM)mode was established.Eight characteristic peptides were selected,and their MRM parameters were optimized.Furthermore,the performance of this HPLC-MS/MS method in species identification was further evaluated.Experimental results demonstrated that this method exhibited high specificity,enabling accurate differentiation of Salmonella from conventional food-borne pathogens.In a milk matrix,the 50%limit of detection(LOD50)was determined to be 0.35 CFU/25 g.This method was successfully applied to qualitatively identify Salmonellain five artificially contaminated food samples.The method presented here,in contrast to traditional biochemical assays,offered both speed and specificity,providing a novel means for the rapid and accurate identification of Salmonella in food.It held significant implications for the surveillance,prevention,and control of food-borne diseases.
