Preparation of Macroporous Strong Cation Exchange Chromatography Medium by Allyl Activation and Its Application in Protein Separation
10.19756/j.issn.0253-3820.221223
- VernacularTitle:烯丙基活化制备大孔强阳离子交换介质及其在蛋白分离中的应用
- Author:
Yan-Xiao XUE
1
;
Heng-Yang HOU
;
Pi-Sheng GONG
;
Hai-Bo JIN
;
Lan ZHAO
;
Guang-Xiang HE
;
Rong-Yue ZHANG
Author Information
1. 北京石油化工学院新材料与化工学院,燃料清洁化及高效催化减排技术北京市重点实验室,北京 102617
- Keywords:
Strong cation exchange chromatography medium;
Allyl activation;
Ligand density;
Adsorption performance;
Separation and purification
- From:
Chinese Journal of Analytical Chemistry
2024;52(12):1896-1903
- CountryChina
- Language:Chinese
-
Abstract:
Strong cation exchange mediums have been widely applied in protein purification.In this work,the strong cation exchange chromatography medium was prepared by coupling small molecular ligands through allyl activation commonly used in agarose-based media.The matrix based on the macroporous microspheres was a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate.The effects of reaction factors on the ligand density were detailedly investigated.The ligand density in the prepared medium was controlled in the range of 0.126-0.370 mol/L(wet gel).Its adsorption performance was evaluated with lysozyme as a model protein.The static binding capacity was in the range of 37.0-81.0 g/L.The maxium dynamic binding capacity reached 37.7 g/L and the maximum lysozyme recovery was 98.97%.The strong cation exchange medium obtained here showed low non-specific adsorption and high separation efficiency on lysozyme.This strong cation exchange medium showed a great potential in rapid protein purification.
