A Fluorescent Small Molecule with Excited State Intramolecular Proton Transfer Property for Detection of Cu2+and Cellular Imaging
10.19756/j.issn.0253-3820.231457
- VernacularTitle:一种具有激发态分子内质子转移特性的荧光小分子在铜离子检测及细胞成像中的应用
- Author:
Yan ZHANG
1
;
Yu-Song LU
;
Wu-Ying LIU
;
Ying CAO
;
Li-Na WANG
Author Information
1. 重庆师范大学化学学院,绿色合成与应用重庆市重点实验室,重庆401331
- Keywords:
Aggregation-induced emission;
Excited-state intramolecular proton transfer;
Copper ion;
Detection;
Cellular imaging
- From:
Chinese Journal of Analytical Chemistry
2024;52(11):1726-1734
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescent sensing technology plays a crucial role in rapid detection of substances and research on visual analysis. In this work,a new type of fluorescent molecule,named SA-AP-PPh3,was successfully synthesized through a two-step process,with a structure composed of triphenylphosphine and salicylaldehyde-aniline Schiff base. This molecule showed the nature of excited-state intramolecular proton transfer (ESIPT) and a large Stokes shift. SA-AP-PPh3 had the ability to selectively detect Cu2+,with a detection range from 0.5 to 15μmol/L and a detection limit as low as 261 nmol/L,which could be used to determine the concentration of Cu2+in actual water samples. In addition,the molecule contained a mitochondrial targeting functional group and had good biocompatibility,which could achieve the visualization of cellular mitochondria and effective detection of Cu2+in cells.
