Epithelial-mesenchymal transition and TGF-β1/Smad signaling in chronic rhinosinusitis and the correlation with surgical prognosis
10.16066/j.1672-7002.2025.09.008
- VernacularTitle:慢性鼻窦炎上皮间质转化与TGF-β1/Smad通路表达及手术预后的相关性研究
- Author:
Yuanzhen SHI
1
;
Yuan HOU
;
Longyan LIU
;
Yong LI
;
Xingjian CHEN
;
Zengping LIU
;
Shihong DUAN
Author Information
1. 兰州大学第二医院(第二临床医学院)耳鼻咽喉头颈外科,甘肃 兰州 730030
- Keywords:
Sinusitis;
Nasal Polyps;
Transforming Growth Factor beta1;
epithelial mesenchymal transition
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2025;32(9):579-584
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare the expression characteristics of transforming growth factor-β1(TGF-β1),Smad2,Smad3,and epithelial-mesenchymal transition(EMT)-related markers(E-cadherin,N-cadherin,vimentin)in patients with different types of chronic sinusitis(CRS),to analyze the correlations of E-cadherin,N-cadherin and vimentin with TGF-β1 and the prognosis of surgical treatment in patients with different types of CRS.METHODS The expressions of E-cadherin,N-cadherin,vimentin,TGF-β1,Smad2 and Smad3 in patients with different types of CRS and the control group were compared by Western blotting(WB)and real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).Analyze its correlation with the improvement degree of each clinical score after the operation.RESULTS Compared with the control group,the expression of E-cadherin decreased in the CRSsNP group,the non-ECRSwNP group and the ECRSwNP group,while the expressions of vimentin and N-cadherin increased.The protein expression of TGF-β1 in the CRSsNP group was higher than that in the non-ECRSwNP group and the control group(P<0.001),and the expressions of Smad2 and Smad3 in the CRSsNP group were higher than those in the ECRSwNP group,the non-ECRSwNP group and the control group(P<0.001).In the CRSsNP group,there was a positive correlation between TGF-β1 and vimentin(r=0.675,P=0.011),and a negative correlation with E-cadherin(r=-0.802,P=0.001).The expression of E-cadherin was negatively correlated with the improvement amplitude of SNOT-22 nasal symptom scores in patients with different types of CRS(P<0.05).CONCLUSION The EMT phenomenon occurs in different types of CRS.In CRS SNPS,EMT may be related to the TGF-β1/Smad signaling pathway.The expressions of EMT markers E-cadherin,N-cadherin and vimentin are correlated with the decrease in the severity of postoperative disease in patients with CRS,suggesting a potential association between the EMT process and the surgical prognosis of patients with CRS.
