Comparison of bacterial clearance protocols in mouse nasal cavities
10.16066/j.1672-7002.2025.08.006
- VernacularTitle:小鼠鼻腔细菌清扫方案比较
- Author:
Yimin REN
1
;
Chengshuo WANG
;
Xiangyi LIU
;
Ying LI
;
Shuang LIANG
;
Gui ZHANG
;
Bing YAN
Author Information
1. 首都医科大学附属北京朝阳医院耳鼻咽喉头颈外科,北京 100020;首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京市耳鼻咽喉科研究所,过敏性疾病北京实验室,慢性鼻病新药及诊断技术研发北京市重点实验室,教育部耳鼻咽喉头颈外科重点实验室,首都医科大学,北京 100005
- Keywords:
Mice;
Nasal cavity;
intranasal;
oral gavage;
antibiotics
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2025;32(8):502-506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effectiveness of different bacterial cleaning methods and their effects on the general condition,nasal mucosal and systemic inflammation of mice.METHODS A total of 44 mice were randomly divided into six groups:4-antibiotic per os group(4ABX po)with 7 mice,5-antibiotic oral gavage group(5ABX og)with 8 mice,5-antibiotic intranasal group(5ABX in)with 8 mice,and each of their control groups with 7 mice.Body weight,water intake,and peripheral blood routine test of mice were measured.Bacterial culture of nasal lavage fluid(NLF)was performed;mRNA level of inflammatory mediators and histopathological analysis were conducted with mouse nasal mucosa.RESULTS Bacteria were cultured from all control groups,while Bacteria were cultured from all control groups,while one mouse in the 4ABX po group,three mice in the 5ABX og group,and two mice in the 5ABX in group showed no bacterial growth.The number of goblet cells in the nasal mucosa significantly increased in the 5ABX og group compared with its control group(P<0.05).The 5ABX in group exhibited significantly higher counts of peripheral blood lymphocytes and hemoglobin levels,as well as greater nasal mucosal thickness compared with its control group,with a notable decrease in goblet cells(P<0.05).No statistical differences were observed in body weight or the mRNA expression of nasal mucosal inflammatory mediators.CONCLUSION Different combinations of antibiotics and administration routes have varying effects on nasal bacteria,systemic and nasal mucosal inflammation in mice.Therefore,choosing appropriate protocols is crucial for the progression of subsequent research.
