Development and evaluation of the clinical standard operating procedure for dust mite nasal allergen challenge
10.16066/j.1672-7002.2025.08.002
- VernacularTitle:尘螨鼻激发试验临床标准化操作流程的建立及评价
- Author:
Yu ZHANG
1
;
Yu SONG
;
Xu ZHANG
;
Jingyun LI
;
Lin XI
;
Yuan ZHANG
;
Luo ZHANG
Author Information
1. 首都医科大学附属北京同仁医院变态反应(鼻过敏)科,北京 100730;首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京 100730;北京市耳鼻咽喉科研究所,过敏性疾病北京实验室,北京 100005
- Keywords:
Dermatophagoides pteronyssinus;
Dermatophagoides farinae;
Rhinitis,Allergic;
Diagnostic Techniques and Procedures;
nasal allergen challenge
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2025;32(8):480-485
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish standardized operating procedures,result interpretation guidelines,and clinical application protocols for dust mite-nasal allergen challenge(NAC),and to systematically evaluate its operational reliability and safety.METHODS Patients with positive serum-specific IgE(sIgE)against mites and healthy controls were enrolled between January 2023 and December 2024.Demographic and clinical information was systematically collected.A standardized mite allergen extract was utilized to prepare the challenge solution for all participants.Bilateral synchronous nasal spray challenges were conducted using progressively increasing concentrations(1 000,3 000,10 000,and 30 000 BU/ml).During the challenge,total nasal symptom scores,visual analogue scale,acoustic rhinometry,active anterior rhinomanometry,and four-phase rhinomanometry were assessed,and all evaluation data and diagnostic outcomes were documented accordingly.RESULTS Compared to the baseline measurements,both subjective symptom scores and objective evaluation indicators in patients demonstrated statistically significant changes following NAC(P≤0.001).Among these patients,40 individuals(81.6%)exhibited a positive response to the dust mite-NAC.All healthy control subjects showed negative results in the test.CONCLUSION This study established standardized operating procedures and clinical application guidelines for the dust mite-NAC.These encompass indications,contraindications,preparation of stimulation solutions,procedural steps,operational standards,criteria for positive results,and pre-/post-examination precautions,thereby providing a clinically actionable framework for dust mite-NAC implementation.
