Influence of inflammatory cytokines related to different endotypes on the transcriptome of nasal epithelial cells
10.16066/j.1672-7002.2025.07.011
- VernacularTitle:不同炎症内在型细胞因子对鼻黏膜上皮细胞转录组的影响
- Author:
Jingjing GUO
1
;
Zhou YU
;
Yuanyuan QIN
;
Zhiqiu ZHU
;
Ming WANG
Author Information
1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京市耳鼻咽喉科研究所,慢性鼻病新药及诊断技术研发北京市重点实验室,过敏性疾病北京实验室,北京 100730
- Keywords:
Sinusitis;
Endophenotypes;
Interferon-gamma;
Interleukin-4;
Interleukin-17;
Transcriptome
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2025;32(7):455-459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the impact of inflammatory cytokines related to different endotypes of chronic rhinosinusitis on the transcriptome of nasal epithelial cells.METHODS Human primary nasal epithelial cells were cultured in vitro,and treated with type 1,type 2,and type 3 inflammatory cytokines IFN-γ(10 ng/ml),IL-4(50 ng/ml),and IL-17A(50 ng/ml),respectively.After 48 hours,cells were collected for RNA extraction and transcriptome analysis.Differentially expressed genes were identified,followed by functional annotation and pathway enrichment analysis.Additionally,common regulated genes among different cytokines were analyzed.RESULTS Treatment of primary nasal epithelial cells with IFN-γ,IL-4,and IL-17A induced significant transcriptomic alterations,with 650,540,and 192 differentially expressed genes,respectively,compared to the control group.Functional annotation and pathway enrichment analysis showed that IFN-γ was mainly associated with defense response to virus,antigen processing and presentation,leukocyte and lymphocyte mediated immunity,and NOD-like receptor signaling pathway;IL-4 was mainly related to the regulation of leukocyte chemotaxis,cell-cell adhesion,ECM-receptor interaction,and MAPK signaling pathway;IL-17A was involved in the regulation of neutrophil chemotaxis,response to molecule of bacterial origin,chemokine signaling pathway,and NF-κB signaling pathway.Genes commonly regulated by IFN-γ,IL-4,and IL-17A were identified,with DUOX2 being upregulated by all the three cytokines.CONCLUSION IFN-γ,IL-4,and IL-17A reshapes the transcriptomic profile of nasal epithelial cells,which mainly regulates signaling pathways related to defense response to virus,leukocyte chemotaxis,and response to bacteria,respectively.
