Immunological characteristics of a mouse model of eosinophilic chronic rhinosinusitis with nasal polyps
10.16066/j.1672-7002.2025.06.006
- VernacularTitle:嗜酸型慢性鼻-鼻窦炎伴鼻息肉小鼠模型免疫学特征表现
- Author:
Yan LI
1
;
Tianjiao JIANG
;
Zhennan QU
;
Luo ZHANG
;
Feng LAN
Author Information
1. 北京市耳鼻咽喉科研究所,慢性鼻病新药及诊断技术研发北京市重点实验室,北京 100005
- Keywords:
Sinusitis;
Nasal Polyps;
Disease Models,Animal;
immunological characteristics
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2025;32(6):366-370
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Aimed to comprehensively evaluate various immunological changes in a mouse model of eosinophilic chronic rhinosinusitis with nasal polyps(ECRSwNP)induced by ovalbumin(OVA)combined with Aspergillus protease(AP),including nasal polyps-like lesions,subepithelial collagen deposition,inflammatory cell infiltration,epithelial barrier function,and olfactory neuron damage.METHODS C57BL/6 mice were challenged with OVA and AP for 12 weeks.Hematoxylin-eosin(HE),periodic acid-Schiff(PAS),and Masson staining were used to observe the changes of nasal polyps-like lesions,goblet cell hyperplasia,and subepithelial collagen deposition.Immunohistochemistry staining(IHC)was employed to detect the changes of various inflammatory cells,olfactory neurons,and the expression of epithelial tight junction proteins in the nasal and sinus mucosa.RESULTS Compared to the control group,the OVA and AP group showed significantly increased epithelial thickness,noticeable nasal polyps-like lesions,marked subepithelial collagen deposition,and goblet cell hyperplasia in the nasal and sinus mucosa.IHC results revealed a significant increase in eosinophils,along with higher numbers of neutrophils,mast cells,and CD4+T cells in the OVA and AP group.Additionally,the expression of tight junction proteins ZO-1 and E-cadherin in the nasal and sinus mucosa and the area of OMP+olfactory neurons in the olfactory epithelium were significantly lower in the OVA and AP group compared to the control.CONCLUSION Continuous exposure to OVA combined with AP successfully induces ECRSwNP.The establishment of this model provides a foundation for further research into the pathogenesis and the evaluation of new therapeutic strategies for ECRSwNP.
