The expression and downstream pathways of miR-21-5p in nasal type 2 inflammation
10.16066/j.1672-7002.2025.04.007
- VernacularTitle:miR-21-5p在鼻黏膜2型炎症中的表达及下游调控通路研究
- Author:
Ming WANG
1
;
Ge LUAN
;
Jing YUAN
;
Chengshuo WANG
;
Luo ZHANG
Author Information
1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京市耳鼻咽喉科研究所,慢性鼻病新药及诊断技术研发北京市重点实验室,过敏性疾病北京实验室,北京 100730
- Keywords:
Sinusitis;
Nasal Polyps;
Rhinitis,Allergic;
type 2 inflammation;
miR-21-5p
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2025;32(4):234-238
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the expression,regulation,and downstream pathways of miR-21-5p in nasal type 2 inflammatory diseases.METHODS The expression levels of miR-21-5p were evaluated in nasal mucosa of patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and healthy controls,as well as in nasal mucosa of CRSwNP and allergic rhinitis(AR)mouse models by real-time PCR.Human primary nasal epithelial cells were cultured and treated with different cytokines.The expression of miR-21-5p were then detected after 48 hours treatment.miR-21-5p lentivirus were used for overexpression in nasal epithelial cells.After 72 hours,cells were collected,and RNA was extracted for transcriptome analysis.Differentially expressed genes were further analyzed for pathway enrichment.RESULTS Compared with healthy controls,the expression of miR-21-5p was significantly increased in the nasal mucosa of CRSwNP patients(P<0.01).The expression of miR-21-5p was also significantly increased in mouse models of CRSwNP and AR respectively,compared to the control groups(P<0.01,respectively).The type 2 inflammation cytokines IL-4 and IL-13 can significantly upregulate miR-21-5p expression in nasal epithelial cells(P<0.01,respectively).Overexpression by lentivirus can significantly increase the expression of miR-21-5p in nasal epithelial cells,and lead to 118 differentially expressed genes.Those genes are significantly enriched in pathways associated with posttranscriptional gene silencing,WNT signaling and MAPKinase signaling.CONCLUSION miR-21-5p is upregulated in nasal type 2 inflammatory diseases partially controlled by IL-4 and IL-13.Overexpression of miR-21-5p lead to the changes of downstream pathways associated with WNT signaling and MAPKinase signaling in nasal epithelial cells.
