Effects of moderate-intensity noise on hearing and basilar membrane macrophages in CBA/CaJ mice
10.16066/j.1672-7002.2024.12.004
- VernacularTitle:中等强度噪声对CBA/CaJ小鼠听力及基底膜巨噬细胞的影响研究
- Author:
Pengfei LIU
1
;
Celia ZHANG
;
Yuan WANG
;
Jiong HU
;
Shuo WANG
Author Information
1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京市耳鼻咽喉科研究所,北京 100730
- Keywords:
Hearing Loss,Noise-Induced;
Evoked Potentials,Auditory,Brain Stem;
Hair Cells,Auditory;
Macrophages
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2024;31(12):765-769
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Observation of audiologic,hair cell,and basilar membrane macrophage changes caused by noise exposure.METHODS Eighteen two-month-old CBA/CaJ mice were randomly divided into three groups:control group,exposure group,and recovery group.The mice were exposed to 100 dB SPL white noise continuously for 7 days,8 hours each day.Hearing was measured at baseline,after exposure,and 60 days post-recovery.After staining,the number,roundness,and area of macrophages in the basal,middle,and apical turns were quantified.RESULTS 1.Under steady-state conditions,the number of macrophages in the basal turn was greater than in the middle and apical turns(F=4.594,P<0.05);the roundness gradually decreased from the basal to the apical turn(F=41.4,P<0.01).2.After noise exposure,there was a significant increase in thresholds across all frequencies(P<0.05),with no hair cell loss observed;60 days post-exposure,thresholds returned to near baseline levels except for the high-frequency range(P<0.05).3.Following exposure,the number and roundness of macrophages increased(P<0.05),while the area decreased(P<0.05).By 60 days post-exposure,these parameters had largely returned to baseline levels.CONCLUSION Under steady-state conditions,macrophages in different regions of the basilar membrane perform distinct functions.Following long-term exposure to moderate-intensity noise,a large number of macrophages are recruited and activated;by 60 days post-exposure,these macrophages have largely returned to their baseline state.
