ORF1p promotes proliferation and invasion of esophageal squamous cell carcinoma cells by regulating AJUBA expression
10.16016/j.2097-0927.202504036
- VernacularTitle:ORF1p通过调控AJUBA表达促进食管鳞状细胞癌细胞增殖和侵袭的研究
- Author:
Fan YANG
1
;
Jiangyang LI
;
Xiaoyan DAI
;
He XIAO
;
Yang PENG
;
Xueling TONG
;
Nan DAI
;
Mengxia LI
Author Information
1. 陆军特色医学中心(第三军医大学大坪医院)肿瘤科
- Keywords:
long interspersed nuclear element-1;
open reading frame 1 protein;
esophageal squamous cell carcinoma cells;
proliferation;
AJUBA
- From:
Journal of Army Medical University
2025;47(13):1429-1443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of open reading frame 1 protein(ORF1p),encoded by long interspersed nuclear element-1(LINE-1),on the proliferation,migration,and invasion of esophageal squamous cell carcinoma(ESCC)cells,and explore the underlying molecular mechanism.Methods① Western blotting was performed to compare the expression of ORF1p between normal esophageal squamous epithelial cells and ESCC cells.② Immunohistochemistry(IHC)assay was used to examine ORF1p expression in ESCC tissues and paired normal tissues adjacent to tumor.③ The effects of ORF1p knockdown and overexpression on malignant behaviors in ESCC cells were determined through functional assays.④ Xenograft tumor model in nude mice was established to evaluate the impact of ORF1p on tumor growth in vivo.⑤ Transcriptome sequencing combined with cell functional rescue experiments were conducted to identify downstream targets regulated by ORF1p.Results ① Western blot analysis demonstrated the expression of ORF1p was significantly higher in the ESCC cell lines than the normal esophageal squamous epithelial cells(P<0.05).② IHC confirmed remarkable up-regulation of ORF1p in ESCC tissues than paired adjacent normal tissues(P<0.000 1).③ Functional assays and experiments on xenograft tumor models revealed that ORF1p substantially enhanced the proliferation,migration,and invasion of ESCC cells,as well as tumorigenic potential in vivo(P<0.05).④ Functional rescue experiments showed that ORF1p facilitated the proliferation,migration,and invasion of ESCC cells by modulating AJUBA expression(P<0.05).Conclusion ORF1p is significantly up-regulated in ESCC and promotes the proliferation,migration,and invasion of ESCC cells by regulating AJUBA expression.
