Hypoxia promotes proliferation of alveolar epithelial cells by regulating chromatin H2A.Z deposition through TAZ-H2A.Z axis
10.16016/j.2097-0927.202502051
- VernacularTitle:缺氧通过TAZ-H2A.Z轴调控H2A.Z染色质沉积促进肺泡上皮细胞增殖的研究
- Author:
Paiyu LIU
1
;
Mengjie ZHANG
;
Jitao ZENG
;
Bing NI
Author Information
1. 陆军军医大学(第三军医大学)高原军事医学系病理生理学教研室,重庆;中国人民解放军联勤保障部队第991医院精神心理科,湖北襄阳
- Keywords:
hypoxia;
alveolar epithelial cells;
TAZ;
histone variant of histone H2A;
proliferation
- From:
Journal of Army Medical University
2025;47(6):498-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and underlying mechanism of the transcriptional coactivator with PDZ-binding motif(TAZ)and a histone variant of acute histone H2A(H2A.Z)in the repair of hypoxia-induced lung injury.Methods A mouse model of hypoxic lung injury was established by being placed in a hypoxia chamber(simulating an altitude of 5 800 m)for 4 d.HE staining was used to observe the severity of lung injury.A hypoxia model of murine alveolar epithelial cells(murine lung epithelial-12,MLE12)was constructed by treating the cells in a hypoxia workstation(1%O2 concentration)for 24 h.Western blotting was employed to detect TAZ expression.The proliferation of alveolar epithelial cells(AECs)was evaluated by CCK-8 assay.Co-immunoprecipitation(Co-IP)assay was utilized to verify the interaction between TAZ and H2A.Z.CUT&Tag sequencing was performed to determine the effect of TAZ on the chromatin deposition of H2A.Z.Results Hypoxia significantly induced alveolar atrophy and inflammatory infiltration in mouse lung tissues(P＜0.01).Hypoxia significantly up-regulated the protein level of TAZ in MLE12 cells(P＜0.05).CCK-8 assay showed that knockdown of TAZ significantly reduced the proliferative capacity of AECs(P＜0.01).Co-IP assay confirmed the physical interaction between TAZ and H2A.Z.CUT&Tag sequencing revealed that hypoxia promoted the deposition of H2A.Z on chromatin(31 817 peaks under normoxia,44 078 peaks under hypoxia),which was partially reversed by TAZ knockdown(37 840 peaks).Conclusion Hypoxia significantly up-regulates the expression of TAZ,which combines with H2A.Z and promotes the deposition of H2A.Z on chromatin,thus enhancing the proliferation of AECs in response to hypoxic injury.
