Effect of sleep deprivation on firing activity of hippocampal parvalbumin-expressing interneurons associated with trace eyeblink conditioning
10.16016/j.2097-0927.202501016
- VernacularTitle:睡眠剥夺对海马PV中间神经元痕迹性眨眼条件反射相关放电活动的影响
- Author:
Yue ZHAN
1
;
Rongrong LI
;
Jie ZHANG
;
Hongli LI
Author Information
1. 陆军军医大学基础医学院:基础医学教学实验中心;陆军军医大学基础医学院:组织胚胎学教研室
- Keywords:
sleep deprivation;
hippocampus;
interneuron;
trace conditioned reflex
- From:
Journal of Army Medical University
2025;47(4):307-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sleep deprivation on the firing activity of hippocampal parvalbumin-expressing interneuron(PV-IN)of mice during trace eyeblink conditioning(tEBC)training and during recovery sleep.Methods A total of 15 PV-Cre mice were randomly assigned into a sleep deprivation group(n=8)and a control group(n=7).After daily tEBC training,the sleep deprivation group experienced 1.5 h of sleep deprivation followed by 1.5 h of recovery sleep,and the control group mice were allowed to sleep freely,for 5 consecutive days.Multichannel recording and optogenetic techniques was applied to identify the hippocampal PV-IN in vivo.Results The sleep deprived mice failed to show significant increase in the incidence of conditioned eyeblink response(CR)across 5 training days(P>0.05).On training days 3～5,the CR incidence was significantly lower in the sleep deprived mice than the control mice(P<0.05).The enhancement of conditioned stimulus(CS)-evoked firing activity in hippocampal PV-INs during tEBC training was significantly lower in the sleep deprived mice when compared to the control mice(P<0.05).The sleep deprived mice obtained notably strengthened firing activity of hippocampal PV-INs peri sharp wave ripple events during recovery sleep period when compared with the pre-training sleep period(P<0.05),and the enhancement was remarkably greater than that in the control mice(P<0.05).Sleep deprivation had no obvious effects on the firing activity of hippocampal non PV-IN during either tEBC training or recovery sleep(P>0.05).Conclusion Sleep deprivation produces dual effects on the firing activities of hippocampal PV-INs during tEBC training and recovery sleep.These effects may be the cellular mechanisms of tEBC memory formation and consolidation disorders caused by sleep deprivation.
