Effect of vagus nerve stimulation on secondary demyelination in rats with acute ischemic stroke
10.16781/j.CN31-2187/R.20240541
- VernacularTitle:迷走神经电刺激对急性缺血性脑卒中大鼠继发性脱髓鞘的影响
- Author:
Sisi WANG
1
;
Furong ZHENG
;
Sanrong WANG
;
Gongwei JIA
Author Information
1. 重庆医科大学附属第二医院康复科,重庆 400010
- Keywords:
stroke;
ischemia/reperfusion injuries;
vagus nerve stimulation;
corpus callosum;
secondary demyelination
- From:
Academic Journal of Naval Medical University
2025;46(4):466-473
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of myelin sheath in corpus callosum on non-ischemic side after ischemic stroke in rats with cerebral ischemia/reperfusion(I/R)model,and to investigate the effect of vagus nerve stimulation(VNS)on secondary demyelination in I/R model.Methods Forty-two rats were randomly assigned to sham,I/R,or I/R+VNS groups,with 14 rats in each group.Except for the sham group,the rats in other groups were used to construct I/R model by a middle cerebral artery occlusion thread method.In addition,the rats in the I/R+VNS group were given repetitive electrical stimulation of the left vagus nerve for 60 min at 30 min after ischemia occlusion.The cerebral infarction volume was observed by 2,3,5-triphenyltetrazolium chloride staining.The expression levels of myelin basic protein(MBP)and myelin-associated glycoprotein(MAG)in the corpus callosum on the non-ischemic side were detected by Western blotting.The integrity and thickness of myelin sheaths in the corpus callosum on the non-ischemic side were observed by Luxol fast blue staining and transmission electron microscopy,respectively.Results Compared with the sham group,the volume of cerebral infarction in the I/R group was increased(P＜0.01).On the 3rd day after I/R,compared with the sham group,the expression levels of MBP and MAG were significantly decreased(both P＜0.01),the intensity of Luxol fast blue staining was significantly decreased(P＜0.01),and the thickness of the myelin sheath was significantly thinner(g-ratio was increased,P＜0.01)in the corpus callosum on the non-ischemic side.Compared with the I/R group,the expression levels of MBP and MAG in the I/R+VNS group were significantly increased(both P＜0.05),the intensity of Luxol fast blue staining was significantly increased(P＜0.01),and the thickness of myelin sheath was significantly increased(g-ratio was decreased,P＜0.01)in the corpus callosum on the non-ischemic side.Conclusion Secondary demyelination occurs on the non-ischemic side of the rat corpus callosum after ischemic stroke,and VNS can ameliorate the demyelination.
