Protective effects of Wuling capsule on concanavalin A-induced autoimmune hepatitis mice
10.16781/j.CN31-2187/R.20230058
- VernacularTitle:五灵胶囊对刀豆蛋白A诱导自身免疫性肝炎小鼠的保护作用
- Author:
Lin CHEN
1
;
Fan CAO
;
Zhongxing SONG
;
Xin KONG
;
Zhishu TANG
;
Hongna LIU
;
Jianping ZHOU
;
Zhaojun CAO
Author Information
1. 陕西省中药资源产业化协同创新中心,秦药特色资源研究开发国家重点实验室(培育),陕西省创新药物研究中心,陕西中医药大学,咸阳 712083
- Keywords:
Wuling capsule;
concanavalin A;
autoimmune hepatitis;
Toll-like receptor 4;
nuclear factor κB
- From:
Academic Journal of Naval Medical University
2025;46(1):65-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of Wuling capsule on mice with autoimmune hepatitis(AIH).Methods Mice were randomly divided into control group,AIH model group,Wuling capsule low-dose group(0.5 g·kg-1·d-1),Wuling capsule middle-dose group(1.0 g·kg-1·d-1),and Wuling capsule high-dose group(2.0 g·kg-1·d-1),with 10 mice in each group.The Wuling capsule groups were administered with Wuling capsule suspension of different doses orally at a volume of 10 mL/kg once daily;the control group and AIH model group were given the same volume of saline by gavage.After 14 d of administration,mice in the AIH model group and Wuling capsule groups were injected with concanavalin A(20 mg/kg)via the tail vein,and the serum,liver,and spleen were collected 8 h after injection.Serum alanine transaminase(ALT)and aspartate transaminase(AST)levels were measured using an automatic biochemical analyzer;hematoxylin-eosin staining was used to observe the pathological structure of the liver tissue;the contents of interleukin(IL)-6,IL-4,and tumor necrosis factor(TNF)-α in liver were determined by enzyme-linked immunosorbent assay,and quantitative polymerase chain reaction was used to determine the relative mRNA expression levels of IL-6,IL-4,TNF-α,Toll-like receptor 4(TLR4),and nuclear factor κB(NF-κB)in the liver.Fluorescence immunoassay was used to analyze the expression and nuclear translocation of NF-κB p65 protein in the liver.Results Compared with the control group,the AIH model group showed abnormal liver morphology and structure,increased serum ALT and AST levels,increased contents of IL-4,IL-6,and TNF-α in the liver,upregulated mRNA expression levels of IL-6,IL-4,TNF-α,TLR4,and NF-κB in the liver,and increased nuclear entry of NF-κB p65.Wuling capsule significantly improved the pathological structure of the liver in AIH mice,reduced serum ALT and AST levels,decreased the contents of IL-4,IL-6,and TNF-α in the liver and the mRNA expression levels of TLR4,NF-κB,IL-4,IL-6,and inhibited the nuclear activation of NF-κB p65.Conclusion Wuling capsule has significant protective effects on AIH mice,which may be related to the TLR4/NF-κB signaling pathway.
