Application of tNGS in patients with influenza A
10.3969/j.issn.1671-8348.2025.11.022
- VernacularTitle:tNGS在甲型流感患者中的应用
- Author:
Qing SUN
1
;
Ying FENG
;
Yali QIU
;
Guojun ZHENG
Author Information
1. 南京医科大学常州医学中心常州市第三人民医院:临床技能教研室,江苏 常州 213000
- Keywords:
tNGS;
influenza nucleic acid;
influenza A;
pathogens;
diagnosis
- From:
Chongqing Medicine
2025;54(11):2612-2615,2620
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application value of targeted next-generation sequencing(tNGS)in the detection of influenza A virus.Methods A retrospective analysis was conducted on the clinical data of 116 patients suspected of having influenza A at our hospital from October 2024 to February 2025.All patients underwent sputum tNGS and throat swab influenza nucleic acid tests due to fever and pulmonary infection,and the diagnostic efficacy of the two methods was compared.Results Among 116 patients,93 were diagnosed with influenza A.Among these 93 influenza A patients,the detection rate of tNGS was higher than that of in-fluenza nucleic acid testing(P<0.05).The results of tNGS and influenza nucleic acid testing for influenza A showed significant consistency with the confirmed diagnosis(P<0.05).There was a statistically significant difference in tNGS sequence counts between the influenza nucleic acid-positive and negative groups(P<0.05).Twenty-three patients excluded from influenza A diagnosis served as the control group.Comparisons of white blood cell(WBC)counts among the influenza nucleic acid-positive group,negative group,and control group showed statistically significant differences(P<0.05),whereas comparisons of C-reactive protein(CRP)and serum amyloid A(SAA)showed no statistically significant differences(P>0.05).Conclusion tNGS demonstrates significant advantages in detecting influenza A virus.Combined with peripheral blood infection-related in-dicators for comprehensive diagnosis,it may provide a more effective approach for early diagnosis and treatment of influenza virus infectious diseases.
