Dicentrine inhibits lipopolysaccharide-induced inflammatory response by modulating apoptosis in RAW264.7 cells
10.3969/j.issn.1671-8348.2025.11.010
- VernacularTitle:荷包牡丹碱调节RAW264.7细胞凋亡抑制脂多糖诱导的炎症反应
- Author:
Zhihui LUO
1
;
Zhengtang SUN
;
Wenju LI
;
Zhiqiang TANG
Author Information
1. 资阳市中心医院急诊科,四川 资阳 641300
- Keywords:
Dicentrine;
RAW264.7 macrophage cells;
apoptosis;
inflammation;
sepsis
- From:
Chongqing Medicine
2025;54(11):2538-2543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Dicentrine in regulating lipopolysaccharide(LPS)-induced inflammatory responses in RAW264.7 macrophages and its underlying mechanisms.Methods RAW264.7 macropha-ges were cultured and pretreated with different concentrations of Dicentrine(250,500,1 000 μmol/L).An inflam-matory response model was established using LPS stimulation(model group),with a control group receiving no treatment.Cytotoxicity of Dicentrine was evaluated by MTT assay.Nitric oxide(NO)levels in cell culture supernatants were measured using the Griess method.Fluorescence microscopy was employed to observe in-tracellular reactive oxygen species(ROS)accumulation and apoptosis.Finally,mRNA expression levels of in-flammatory cytokines including IL-1β,IL-6,inducible nitric oxide synthase(iNOS),and tumor necrosis factor-α(TNF-α)were analyzed by qPCR.Results MTT assay results indicated that Dicentrine exhibited no cyto-toxic effects at concentrations below 1 000 μmol/L.Griess method and fluorescence microscopy showed signif-icantly elevated NO and ROS levels in the model group compared to the control group(P<0.05).Compared with the model group,the 250,500,and 1 000 μmol/L Dicentrine groups demonstrated significantly reduced NO and ROS levels(P<0.05).qPCR analysis revealed significantly increased mRNA expression of IL-1β,IL-6,iNOS,and TNF-α in the model group versus the control group(P<0.05).Compared with the model group,the 250,500,and 1 000 μmol/L Dicentrine groups showed significantly decreased mRNA expression of IL-1β,IL-6,iNOS,and TNF-α(P<0.05).Conclusion Dicentrine regulates macrophage apoptosis,thereby ef-fectively inhibiting LPS-induced inflammatory responses in macrophages.
