Experimental study on extraction and transplantation of live cells from burn scab tissue combined with artificial dermal scaffold coverage for treating deep Ⅱ-degree burn wounds
10.3969/j.issn.1671-8348.2025.10.008
- VernacularTitle:烧伤痂皮组织活细胞提取回植联合人工真皮支架覆盖治疗深Ⅱ度烧伤创面的实验研究
- Author:
Lei CHEN
1
;
Gaofeng FANG
;
Tesheng GAO
Author Information
1. 嘉兴市中医医院麻醉科,浙江嘉兴 314000
- Keywords:
deep burns;
burn scab tissue;
extraction of live cells;
replanting;
artificial dermis scafold
- From:
Chongqing Medicine
2025;54(10):2282-2288
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of extracting and transplanting the live cells from burn scab tissue combined with artificial dermal scaffold coverage for the treatment of deep Ⅱ-degree burn wounds.Methods Four female Bama miniature pigs aged 7 to 8 months were successfully anesthetized and 3 square skin deep Ⅱ-degree burn wound models with a side length of 50 mm were established on each side of the spine(each wound is divided into four parts of a square with a side length of 25 mm).A total of 24 wounds were established.The ipsilateral wounds were divided into the dermis group and the combination group.After the live cells from the scabs were extracted and replanted on the wound surface of the combined group,an artificial dermal scaffold was covered.The wound surface of the dermis group was simply covered with an artificial dermal scaffold.On 7,14,21,28 d of the experiment,5 mm diameter tissues corresponding to the central part of each wound surface after anesthesia were successively and simultaneously cut by circular drilling.The HE staining was performed to observe the coverage rate of wound epidermis,the Masson staining was used to observe the tissue collagen content,Ki-67 antigen was used to observe the proliferation rate of wound cells,and CD31 count was used to observe the neovascularization of wound capillaries.Results On 7 d of the experiment,no wound epidermis coverage was observed in both groups.The wound coverage rate on 14,21,28 d in the combination group was higher than that in the dermis group,and the difference was statistically significant(P＜0.05),moreover,the wound coverage rate in the combination group was increased with the experimental time was extended(P＜0.05).The collagen content on 7 d of experiment in the combination group was higher than that in the dermis group,and the difference was statistically significant(P＜0.05).The Ki-67 expression level of the wound tissue on 7-28 d in the combination group was higher than that in the dermis group,and the difference was statistically significant(P＜0.05).There was no statistically significant difference in the CD31 expression level during 7-28 d of experiment between the two groups(P＞0.05).Conclusion The extraction and transplantation of live cells from deep burn scab tissue combined with artifi-cial dermal scaffolds coverage could partially repair burn deep Ⅱ-degree wound.
