Effect of Lingzhu Granules on PI3K/Akt signaling pathway in rats with polycystic ovary syndrome accompanied by insulin resistance
10.3969/j.issn.1671-8348.2025.09.001
- VernacularTitle:灵术颗粒对多囊卵巢综合征伴胰岛素抵抗大鼠PI3K/Akt通路的影响
- Author:
Dongyu YANG
1
;
Shan LU
;
Junhong ZHANG
;
Xulong CHEN
;
Sinan LI
;
Yan XIE
Author Information
1. 广州中医药大学第二附属医院/广东省中医院生殖医学科,广州 510120
- Keywords:
polycystic ovary syndrome;
insulin resistance;
Lingzhu Granules;
phosphatidylinositol 3-ki-nase;
protein kinase B
- From:
Chongqing Medicine
2025;54(9):2001-2006
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulatory effect of Lingzhu Granules on the PI3K/Akt signaling pathway in the rats with polycystic ovary syndrome(PCOS)accompanied by insulin resistance.Methods Fe-male Sprague-Dawley(SD)rats aged 3 weeks d old were selected to establish the model by using the DHEA method.The intervention treatment was administered by different doses of Lingzhu Granules and the homeostasis model as-sessment of insulin resistance(HOMA-IR)was calculated.The relative expression levels of PI3K,Akt,GSK-3β and GLUT4 mRNA in the ovaries was detected by RT-PCR.The expression levels of PI3K and Akt were detected by Western blot.Results Compared with the model control group,the rat body weight in the medium and high doses Lingzhu Granules groups was significantly reduced(P＜0.05).The fasting blood glucose,fasting insulin and HOMA-IR index in the Lingzhu Granules low,medium and high doses groups were significantly lower than those in the model control group,and the differences were statistically significant(P＜0.01).The mRNA relative expression levels of PI3K,Akt,GSK-3β and GLUT4 in the Lingzhu Granules medium and high doses groups all were higher than those in the model control group(P＜0.05).The relative expression levels of PI3K and Akt in the ovarian tissue in the Lingzhu Granules low,medium and high doses groups were higher than those in the model control group,and the differences were statistically significant(P＜0.05).Conclusion Lingzhu Granules could effectively improve the insulin resistance status of PCOS rats by enhancing the synthesis of PI3K and Akt.
