Relationship between Klotho,IGF-1,and Irisin in the placentas of pregnant rats and the intrauterine development of fetuses
10.3969/j.issn.1671-8348.2025.06.001
- VernacularTitle:孕鼠胎盘中Klotho、IGF-1及鸢尾素与胎鼠宫内发育的关系
- Author:
Fang ZHANG
1
;
Linlu ZHENG
;
Chan WANG
;
Mengmeng LI
;
Yaying CHENG
Author Information
1. 河北北方学院,河北 张家口 075000;河北省人民医院儿科,石家庄 050051
- Keywords:
placenta;
intrauterine development;
Klotho;
insulin-like growth factor-1;
Irisin
- From:
Chongqing Medicine
2025;54(6):1281-1285,1292
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between Klotho,insulin-like growth factor-1(IGF-1),and Irisin in the placentas of pregnant rats and the intrauterine development of fetuses.Methods Thirty SD rats were divided into the intrauterine growth restriction(IUGR)experimental animal model group(IUGR group),the macrosomia experimental animal model group(macrosomia fetal group),and the control group ac-cording to different feeding diets(low-protein diet,high-protein diet,and normal diet).All pregnant mice were subjected to laparotomy for tissue sampling on the 21st day of pregnancy.The birth weight,body length,and placental mass of fetal rats were recorded.The relative mRNA expression levels of Klotho,IGF-1,and Irisin in the placentas were detected by quantitative real-time fluorescent reverse transcription-PCR(qRT-PCR),and the correlations between various factors above were analyzed.Results Compared with the control group,the birth weight,body length and placental mass of fetal rats in the IUGR group were lower,while those in the macrosomia fetal group were higher,with statistically significant differences(P<0.05).Compared with the control group,the relative mRNA expression levels of Klotho,IGF-1,and Irisin in the IUGR group were de-creased,while those in the macrosomia fetal group were increased,with statistically significant differences(P<0.05).Correlation analysis showed that Klotho mRNA was positively correlated with the relative expres-sion levels of placental IGF-1 mRNA and Irisin mRNA,and IGF-1 mRNA were also positively correlated with the relative expression level of placental Irisin mRNA(P<0.05).Conclusion Klotho,IGF-1,and Irisin in the placenta are associated with fetal growth and possibly regulated through the Klotho/IGF-1/Irisin axis.
