Application value analysis of loop-mediated isothermal amplification technique in neonatal bacterial infectious diseases
10.3969/j.issn.1671-8348.2025.05.023
- VernacularTitle:环介导等温扩增法在新生儿感染性疾病中的应用价值分析
- Author:
Na LI
1
;
Jianbo XU
;
Huijie JIN
Author Information
1. 金华市妇幼保健院检验科,浙江金华 321000
- Keywords:
loop-mediated isothermal amplification;
bacterial cultivation method;
neonatal infection;
methicillin-resistant Staphylococcus;
sensitivity;
specificity
- From:
Chongqing Medicine
2025;54(5):1172-1175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical value of loop-mediated isothermal amplification(LAMP)for rapid detection of neonatal infectious pathogens.Methods Biological samples(cord blood and neonatal blood or sputum within 48 hours of birth)of 167 suspected neonates and premature infants delivered in Jinhua Maternal and Child Health Hospital from June 2021 to December 2022 were collected and tested by LAMP method and bacterial culture method respectively.The results of the two methods were compared consistent-ly.Using the bacterial culture method as the standard,the positive rate of LAMP method was evaluated in de-tecting common pathogens in neonatal infections.Results Among the 167 specimens,the positive rate of pathogen detection by LAMP method(24.0％)was higher than that by bacterial culture method(15.0％),and the difference was statistically significant(x2=4.30,P＜0.05).The positive rate of LAMP method for detecting methicillin-resistant Staphylococcus aureus was higher than that of bacterial culture method,and the difference was statistically significant(x2=15.59,P＜0.01).Conclusion Compared with bacterial culture method,LAMP method has a higher positive rate for detecting methicillin-resistant Staphylococcus aureus,and can quickly and accurately diagnose the pathogenic bacteria of neonatal infections,providing a basis for the rapid development of clinical diagnosis and treatment strategies.
